复方血栓通胶囊HPLC指纹图谱质量控制方法研究  

HPLC Fingerprint of Compound Xueshuantong Capsule

作　　者：梁洁萍[1] 刘忠政[1] 彭维[1] 苏薇薇[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中药材》2012年第11期1854-1858,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:建立复方血栓通胶囊HPLC指纹图谱,为评价其质量提供依据。方法:色谱柱为Dionex Acclaim120 C18(4.6 mm×150 mm,3μm);以乙腈(A)-0.05%磷酸溶液(B)为流动相,梯度洗脱,洗脱程序如下:0～50 min(15%→34%A),50～95 min(34%→75%A);检测波长为203、270 nm;流速1.0 mL/min;柱温25℃。结果:该指纹图谱中,确定了42个共有峰,可检出复方血栓通胶囊中4味药材;并采用RRLC-MS/MS及HPLC-DAD确证和指认了指纹图谱中23个化学成分。结论:该方法操作简便、准确可靠、重复性较好,为复方血栓通胶囊的质量控制提供了有效手段。Objective：To establish the HPLC fingerprint of Compound Xueshuantong Capsule. Methods：Dionex Acclaim 120 C18 column（4. 6 mm× 150 mm,3 μm） was used with acetonitrile（ A）and 0. 05% phosphoric acid（B） in gradient elution mode. The elution profile was ：0 - 50 min（ 15%→34% A）,50 - 95 min（34%→75% A） ;The detective wavelength was 203 nm and 270 nm. The column temperature was set at 25 ℃ and the flow rate was 1.0 mL/min. Results： In the established fingerprint,42 common peaks covering 4 medicinal materials were detected, and 23 chemical compounds were identified by RRLC/MS/MS and DAD spetra, Conclusion： The method can be used for quality control of Compound Xueshuantong Capsule with great precision, accuracy and good reproducibility.

关 键 词：复方血栓通胶囊 高效液相色谱法 指纹图谱 

分 类 号：R286.02[医药卫生—中药学]