猪传染性胸膜肺炎放线杆菌变异株的分离与鉴定  

Isolation and identification of a Actinobacillus pleuropneumoniae variant strain

作　　者：葛兆宏[1] 陆广富[1] 陶洁[2] 张志妮[2] 何玲[2] 朱国强[2] 

机构地区：[1]江苏畜牧兽医职业技术学院,江苏泰州225300 [2]扬州大学兽医学院,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2012年第4期10-13,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家"十一五"科技支撑计划项目(2006BAD06A12)

摘　　要：对江苏泰兴某猪场疑似猪传染性胸膜肺炎病料进行实验室分离培养,成功分离到1株猪传染性胸膜肺炎放线杆菌,并对其进行生化特性、双抗夹心ELISA鉴定。为进一步分析该分离株的血清型,针对ApxIVA和ApfA保守基因设计2对检测引物,PCR结果表明:该分离株未扩增出预期大小的片段,说明该分离株很有可能是1株猪传染性胸膜肺炎放线杆菌变异株,其血清型还有待进一步鉴定。In this study, an Actinobacillus pleuropneurnoniae strain was isolated from the sick pig in Taixing and its biochemical characteristics were studied. Monoclonal antiboday-based sandwich ELISA was used to detect the isolate. In order to study the serotype of the isolate, two pairs of primers against ApxIVA and ApfA genes were designed and used for PCR detection. The PCR results demonstrated that the target bands of the strain did not appeared, which suggested that the isolate might be an Actinobacillus pleuropneumoniae variant strain and its serotype remains to be further con- firmed.

关 键 词：猪传染性胸膜肺炎放线杆菌 双抗夹心ELISA PCR 

分 类 号：S858.28[农业科学—临床兽医学]