单克隆抗体酶联免疫吸附法检测尿标本中人巨细胞病毒抗原  

Detection of Human Cytomegalovirus Antigen in Urine Samples by Enzyme-linked Immunosorbent Assay

作　　者：张欣[1] 袁丽[2] 智再新[2] 陈秀珠[1] 吴远明[2] 

机构地区：[1]同济医科大学病毒学研究中心,武汉430030 [2]中国科学院武汉病毒研究所,武汉430071

出　　处：《病毒学杂志》1991年第2期102-106,共5页

摘　　要：本文运用抗人巨细胞病毒(HCMV)包膜20KD或/和130KD结构蛋白的单克隆抗体分别建立了4类酶联免疫吸附试验(ELISA)夹心法,共对44人份临床尿标本进行HCMV抗原检测。方法的敏感度可高达10.3—32.8ng HCMV抗原/ml尿,与尿标本中的HSV-Ⅰ、HSV-Ⅱ和EBV抗原无交叉反应,重复性良好,与病毒分离比较,敏感性和特异性在71—83％和88—100％之间;与核酸杂交比较,敏感性和特异性也可分别高达60—100％和83.3—100％。混合使用多种单克隆抗体作为包被抗体会得到较好的技术参数。上述结果提示运用单克隆抗体ELISA将有助于一般临床实验室对HCMV感染的快速诊断。An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of human cytomegalovirus (HCMV) in urine samples There were 4 kinds of method of the ELISA and monoclonal antibody directed against 20 or 130 kilodalton envelope structural polypeptides of HCMV was served as a capture antibody for viral antigen respectively or together,it has also been done positive serum of HCMV specific IgG. The assays were capable of detecting 10—32 ng HCMV antigen/ml and no cross reaction with HSV-Ⅰ, HSV-Ⅱ and EBV antigen in urine sample The assay was found to have sensitivity of 71—83％, 60—100％ andspecificities of 88—100％, 83.3—100％ in comparison with virus isolantion assay and DNA-DNA hybridization assay respectively when 44 thawed urene specimen were examined.

关 键 词：人巨细胞病毒 单克隆抗体 ELISA 

分 类 号：R446.62[医药卫生—诊断学]