天目铁木愈伤组织和芽苗诱导技术  

Technique of callus induction and bud seedling of Ostrya rehderiana Chun

作　　者：顾地周[1] 陆爽[1] 巴春影[1] 李媛媛[1] 

机构地区：[1]通化师范学院生物系,吉林通化134002

出　　处：《浙江大学学报（理学版）》2013年第2期216-220,共5页Journal of Zhejiang University（Science Edition）

基　　金：国家科技部国家科技攻关计划引导项目(2005BA741C);吉林省科技厅基金资助项目(200705C05)

摘　　要：以天目铁木嫩茎尖为外植体,应用均匀设计法筛选其基部愈伤组织诱导和愈伤组织再分化芽苗的最适合培养基.结果表明,最适合的嫩茎尖基部愈伤组织诱导培养基为1/2DR+TDZ 2.30 mg.L-1+2,4-D 0.55mg.L-1,诱导率为93.5%;愈伤组织芽苗再分化培养基为1/2DR+TDZ 3.30mg.L-1+KT 0.70mg.L-1,分化率达99.8%以上.成功建立了天目铁木嫩茎尖离体诱导愈伤组织和芽苗再分化体系,且再生芽苗与野生植株染色体数目相同.The tender stem tips of Ostrya rehderiana Chun was used as explant and its suitable medium compositions were screened for callus induction and shoots redifferentiation through uniform design experiments. The results show that the most suitable culture mediums were as follows： 1/2DR＋TDZ 2.30 mg·L^-1 ＋2,4-D 0.55 mg·L^-1 for callus induction at base of tender stem tip, the rate of induction was 93.5·L^-1/2DR＋TDZ 3.30 mg ·L^-1 ＋KT 0.70 mg·L^-1for shoots redifferentiation, the rate of differentiation was more than 99.8%. Callus induction and shoots redifferentiation in vitro system of Ostrya rehderiana Chun have been successfully established, and regenera- tion buds seedlings and wild plant have the same chromosome number.

关 键 词：天目铁木 愈伤组织 诱导 再分化 均匀设计 

分 类 号：Q945.5[生物学—植物学] Q949.736.2