香菇135和9015品种遗传多样性的RAPD分子标记研究  

Study on the genetic diversity of Lentinula edodes 135 and 9015 by random amplified polymorphic DNA(RAPD) markers

作　　者：叶长文[1] 谭海芹[2] 吴应淼[1] 吴敏[2] 朱旭芬[2] 

机构地区：[1]浙江省庆元县食用菌科研中心,浙江省食用菌开发中试重点试验室,浙江丽水323000 [2]浙江大学生命科学学院,浙江杭州310058

出　　处：《浙江大学学报（理学版）》2013年第2期230-234,共5页Journal of Zhejiang University（Science Edition）

基　　金：浙江省食用菌新品种选育重大科技专项(2012C12911)

摘　　要：通过抗逆性较差的香菇135品种和自然变异抗性新菌株庆元9015品种的RAPD研究,寻找两者间有差异的基因,并对其进行克隆和测序.GenBank查询结果表明,这些片段可能的部分开放阅读框(ORF)与多种生物的水解酶或者信息素受体的部分氨基酸序列有较高的同源性.这些片段可以作为两者间的特异分子标记.Random amplified polymorphic DNA （RAPD）was used to study the differencial genes between the Lentinula edodes （Qingyuan） strain 135 with poor resistance and strain 9015 （a new strain by natural variation）. Several differencial genes were obtained, and then cloned and sequenced. The results in GenBank revealed that the partially possible （）pen Reading Frames （ORF） of these fragments had higher homology with some hydrolases and pheromone precursor receptor genes of several kinds of organisms. And we presume these genes fragments could be as the specific molecular markers for specifying Lentinula edodes 135 and 9015.

关 键 词：香菇135 香菇9015 RAPD 水解酶 信息素受体 

分 类 号：S646.12[农业科学—蔬菜学]