响应面法优化解淀粉芽孢杆菌D1产抗菌素培养基研究  

Optimization of Fermentation for Production Conditions of Antibiotic by Bacillus amyloliquefaciens D1

作　　者：吕钊[1,2] 张丽萍[1,3] 程辉彩[1,3] 麻耀华[1] 习彦花[1] 

机构地区：[1]河北省科学院生物研究所,河北石家庄050081 [2]河北师范大学生命科学学院,河北石家庄050016 [3]河北省主要农作物病害微生物控制工程技术研究中心,河北石家庄050081

出　　处：《北方园艺》2013年第5期94-97,共4页Northern Horticulture

基　　金：国家农业成果转化基金资助项目(2010GB2A200041);河北省科技支撑计划资助项目(10205520D);河北省科学院重大攻关计划资助项目(12306)

摘　　要：以1株解淀粉芽孢杆菌为出发菌,采用Box-Behnken试验及响应面分析相结合的方法对其产抗菌素的发酵培养基进行优化。结果表明:最佳培养基配方为C源淀粉1.88%,N源牛肉膏1.76%,酵母膏0.74%,KH2PO40.25%,K2HPO40.1%,(NH4)2SO40.08%。通过优化产抗菌素培养基,抗菌素的抑菌圈直径由优化前的19.2mm提高到优化后的27.2mm,提高了41.7%。The Box-Behnken experiment and response surface analysis method were combined to optimize the fermentation medium of a Bacillus amyloliquefaciens. The results showed that the optimal fermentation medium formula consisted of 1.88% starch,1.76% beef extract,0. 74% yeast extract, KH2 PO4 0. 25%, K2 HPO4 0. 1%, （NH4）2 SO4 0. 08%. There was a 41.7% increase in the yield of antibacterial peptides obtained using the optimal fermentation medium, compared with that before optimization.

关 键 词：解淀粉芽孢杆菌 响应面分析 Minitab软件 抗菌素 

分 类 号：S482.28[农业科学—农药学]