卫星搭载苜蓿叶片愈伤组织对盐碱胁迫的响应  

Alfalfa Leaf Callus on Space Flight Induction and Response on Salinealkali Stress

作　　者：李红[1] 李波[2] 王烨[2] 王晨[2] 杨伟光[1] 杨曌[1] 

机构地区：[1]黑龙江省畜牧研究所,黑龙江齐齐哈尔161000 [2]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《北方园艺》2013年第5期100-102,共3页Northern Horticulture

基　　金：国家科技支撑计划资助项目(2011BAD17B01-04);国家公益性行业科研专项资助项目(201303066);黑龙江省教育厅科学技术研究资助项目(12521610)

摘　　要：以卫星搭载的苜蓿叶片为外植体,诱导产生愈伤组织,研究盐碱胁迫下苜蓿愈伤组织对盐和碱的半致死浓度和致死浓度,及其生理指标的变化。结果表明:苜蓿愈伤组织对NaCl的半致死浓度为1.2%,致死浓度为1.8%。对NaHCO3和Na2CO3的半致死浓度为0.4mmol/L,致死浓度为1.0mmol/L。卫星搭载提高了苜蓿愈伤组织POD活性、可溶性糖含量和脯氨酸含量,降低了丙二醛含量和相对电导率,差异显著。卫星搭载可以提高苜蓿愈伤组织对盐碱的抵抗能力,且对碱抵抗能力强于对盐的抵抗能力。Taking alfalfa leaves on space flight as materials, the callus was induced. The half lethal concentration and lethal concentration of alfalfa callus under different concentrations of saline and alkali stress were studied, and 5 physiological and biochemical indexes under different saline-alkali stress were studied. The results showed that the half lethal and lethal concentration of alfalfa callus under NaCl stress were 1.2 % and 1.8 mmol/L. The half lethal and lethal concentration of alfalfa callus under NaHCO3 and Na2 CO3 stress were 0. 4 % and 1.0 mmol/L. The Alfalfa callus on space flight improved POD activity, soluble sugar and pro!ine content decreased the MDA content and relative conductivity, and the difference was significant. Space flight could improve saline： alkali ：resistance of alfalfa callus, and alkali resistance was higher than that of salt resistance.

关 键 词：苜蓿 愈伤组织 卫星搭载 盐碱胁迫 生理生化指标 

分 类 号：S551.7[农业科学—作物学]