正交试验优选地龙水提取工艺  

Optimization of Water Extraction Technology for Pheretima aspergillum by Orthogonal Test

作　　者：贺葵邦[1] 王英姿[1] 张春泥[1] 冯艾灵[1] 张胜海[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2013年第6期33-35,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京中医药大学复方中药制药研究创新团队项目(2011-CXTD-13);中药制药过程新技术国家重点实验室开放基金项目(SKL2010M0302);北京中医药大学自主课题(2011-JYBZZ-JS048)

摘　　要：目的:优选地龙的水提取工艺条件。方法:以次黄嘌呤含量为指标,HPLC测定指标成分含量,在单因素试验基础上,采用L9(34)正交试验考察加水量、提取次数、提取时间对次黄嘌呤含量的影响,优选地龙的水提取工艺。结果:各因素对水提取工艺的影响顺序为提取次数>提取时间>加水量;最佳水提取工艺为地龙药材加10倍量水回流提取1次,提取时间1 h。结论:优选的水提取工艺稳定可行。Objective： To optimize water extraction technology conditions of Pheretima aspergillum. Method： With the content of hypoxanthine as index, based on single factor test, effects of extraction time, extraction times and the amount of water were investigated by L9（34） orthogonal test.HPLC was used to determine the content of hypoxanthine. Result： Influencing order of various factors on water extraction technology was extraction times〉extraction time〉the amount of water;Optimal water extraction technology was as following：reflux extracted 1 time with 10 times the amount of water, extraction time of one hour. Conclusion： This optimized technology was stable and feasible.

关 键 词：地龙 提取工艺 单因素试验 正交试验 

分 类 号：R283.6[医药卫生—中药学]