指纹图谱法考察活络效灵丹加减方的不同提取工艺  

Investigation of Different Extraction Technology for Addition and Subtraction Prescription of Huoluo Xiaoling Dan by Fingerprint

作　　者：高文娟[1] 谭雪梅[1] 戴荣华[1] 毕开顺[1] 陈晓辉[1] 

机构地区：[1]沈阳药科大学药学院中药质量控制技术国家地方联合工程实验室,沈阳110016

出　　处：《中国实验方剂学杂志》2013年第6期40-43,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:采用HPLC指纹图谱研究不同提取工艺对活络效灵丹加减方(HLXL)中化学成分变化的影响。方法:以獐牙菜苦苷、芍药苷、隐丹参酮和异欧前胡素等11个特征指纹峰为指标,采用HPLC分析测定,条件为YMC-Pack ODS-A色谱柱,流动相甲醇-水梯度洗脱,检测波长210 nm,流速0.8 mL.min-1,柱温30℃。系统考察不同溶剂(水、乙醇、丙酮)、不同提取方式(超声、回流、冷浸)及单提混合液和混提液对各特征指纹峰相对含量的影响。结果:不同工艺提取物中特征峰相对峰面积存在较大差异。溶剂种类和提取方式对HLXL成分的提取有较大影响。结论:该方法重复性、精密度、稳定性良好,可用于HLXL不同提取工艺的指纹图谱研究。Objective： To study influence of different extraction technology on chemical compositions changes from addition and subtraction prescription of Huoluo Xiaoling Dan（HLXL） by HPLC fingerprint. Method： With swertiamarin, paeoniflorin, cryptotanshinone, isoimperatorin and other seven characteristic fingerprint peaks as index, which were determined by HPLC, with YMC-Pack ODS-A column, methonal-water（gradient elution） as mobile phase, detection wavelength 210 nm, flow rate 0.8 mL·min-1, column temperature 30 ℃.Effects of different solvents（water, ethanol, acetone）, extract models（ultrasound, refluxing, macerating） and mixed-extract and separated-extract on relative content of each characteristic peaks were systematically investigated. Result： Different technology extracts had significant difference on relative peak area of eleven evaluating indexes.Kinds of solvent and extraction types and order of mixing had a great influence upon extraction efficiency of chemical compositions from HLXL. Conclusion： This method was reproducible, precise, stable and could be applied to study on fingerprint of HLXL with different extraction technology.

关 键 词：活络效灵丹加减方 指纹图谱 獐牙菜苦苷 芍药苷 隐丹参酮 异欧前胡素 

分 类 号：R283.6[医药卫生—中药学]