土茯苓配方颗粒HPLC指纹图谱研究  

Study on Fingerprint of Smilacis Glabrae Rhizoma Formula Granules by HPLC

作　　者：温庆伟[1,2] 邹录惠 蓝晓庆 黄丽凤 陆东 韦红言 周永强 

机构地区：[1]广西医科大学药学院,南宁530021 [2]培力(南宁)药业有限公司,南宁530007

出　　处：《中国实验方剂学杂志》2013年第6期99-102,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西千亿元产业重大科技攻关项目(桂科攻11107009-2)

摘　　要：目的:采用反相高效液相色谱法(HPLC),建立土茯苓配方颗粒的指纹图谱。方法:采用UltimateAQ-C18(4.6 mm×250 mm,5μm)色谱柱,甲醇-0.2%磷酸水溶液(35∶65)洗脱;检测波长290 nm;流速1.0 mL.min-1,进样量10μL,采集时间60 min。结果:分析方法有较好的重复性,共检出7个共有峰,并指认出落新妇苷(astilbin)、白藜芦醇(resveratrol)2个化学成分。10批土茯苓配方颗粒的指纹图谱相似度均>0.98。结论:HPLC指纹图谱重复性好,可作为土茯苓配方颗粒产品质量控制的技术手段之一。Objective： To investigate and establish the fingerprint of Smilacis Glabrae Rhizoma Formula Granules by RP-HPLC. Method： The samples were injected with 10 μL in a HPLC system coupled with Ultimate AQ-C18 column（4.6 mm×250 mm, 5 μm）, and eluted with methanol-0.2% phosphoric acid solution（35： 65） at 1.0 mL·min-1 under 30 ℃.The data was recorded for 60min by UV director at 290 nm. Result： Two compounds（astilbin, resveratrol）were identified from the 7 common peaks in the fingerprint which had a good reproducibility and similarity with correlation coefficient （〉0.98） in the analysis of 10 different batches products. Conclusion： HPLC fingerprint would be a selective, simple, acute and suitable method for quality control in the herbs and Formula Granules of Smilacis Glabrae Rhizoma.

关 键 词：土茯苓 配方颗粒 高效液相色谱法(HPLC) 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]