LC-MS-MS法测定参体和参须中人参皂苷Rb_1,Rb_2和Rc含量  

Simultaneous Quantitation of Rb_1,Rb_2 and Rc in Ginseng Tail and the Main Root of Ginseng by LC-MS-MS

作　　者：闫寒[1] 赵松岩[2] 刘运嘉 吴睿凡[4] 范斌[1] 郭娜[1] 彭娟[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700 [2]沈阳药科大学,沈阳110016 [3]沈阳市药品不良反应和医疗器械不良事件监测中心,沈阳110013 [4]新疆医科大学,乌鲁木齐830011

出　　处：《中国实验方剂学杂志》2013年第6期105-107,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院医学实验中心自主课题项目(zz2007002)

摘　　要：目的:建立检测参体和参须中人参皂苷Rb1,Rb2和Rc的LC-MS-MS方法。方法:应用液质联用,C18色谱柱系统,采用梯度洗脱方法,A-水(0.05%FA),B-乙腈,A-B=65∶35,梯度洗脱(0～3 min,65%B;3～4 min,100%B),流速0.35mL.min-1,柱温40℃,建立了参体和参须中人参皂苷Rb1,Rb2和Rc含量测定方法并进行了方法学考察。结果:建立了同时检测参体和参须中人参皂苷Rb1,Rb2和Rc含量测定方法。结论:该方法准确、简便、快速,可用于参体和参须的质量控制。Objective： To develop a method to determine the content-of ginsengnoside Rb1, Rb2 and Rc in ginseng tail and the main root of ginseng by using HPLC-MS-MS. Method： C18 HPLC column was used in the system including HPLC-MS-MS with a gradient elution program.The mobile phase was consisted of： A for water（0.05%FA）, B for acetonitrile, A： B=65： 35; gradient elution program was as follows：0-3 min, 65%（B）; 3-4 min, 100%（B）.The flow rate was 0.35 mL·min-1; column temperature was kept at 40 ℃. Result： Simultaneous quantitation of ginsengnoside Rb1, Rb2 and Rc in ginseng tail and the main root of ginseng by HPLC-MS-MS was developed. Conclusion： The method is accurate, convenient and rapid, which can be applied for the quality control of ginseng tail and the main root of ginseng.

关 键 词：参体和参须 含量测定 人参皂苷RB1 人参皂苷Rc 人参皂苷Rb2 

分 类 号：R284.1[医药卫生—中药学]