HPLC测定匙羹藤花中牛弥菜醇A的含量  

Determination of Conduriol A in the Flower of Gymnema sylvestre

作　　者：丘琴[1] 甄汉深[1] 黄培倩[1] 李文秀[1] 

机构地区：[1]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2013年第6期155-158,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西研究生教育创新计划项目(2008106001007M09);广西教育厅科研项目(桂教科2001);广西中医学院重点科研项目(ZD2004010);广西自然科学基金项目(桂科自0229044)

摘　　要：目的:对匙羹藤花中的有效成分牛弥菜醇A的含量测定进行研究,为科学评价其质量提供科学依据。方法:采用正交实验优选匙羹藤花样品溶液的制备方法,同时采用高效液相色谱法(HPLC)测定牛弥菜醇A的含量,以氨基柱(4.6 mm×250 mm,5μm)为色谱柱,乙腈-水(90∶10)为流动相,流速1.0 mL.min-1,检测波长207 nm,柱温:30℃。结果:匙羹藤花样品溶液的最佳制备方法为150倍50%甲醇超声提取50 min,牛弥菜醇A在0.400 4～20.02μg与峰面积成良好的线性关系(r=0.999 7),平均回收率为98.98%,RSD 1.15%(n=6)。结论:采用HPLC建立的方法准确、快速、灵敏度高、重复性好,可作为匙羹藤花的质量控制方法。Objective： To study the determination of conduritol A in the flower of Gymnema sylvestre. Method： The best extraction technology for conduritol A was studied through orthogonal experiment; HPLC was adopted to determine the content of conduritol A.The separation was performed on a Thermo NH2 column（4.6 mm×250 mm, 5 μm）; acetonitrile：water（90： 10）was used as mobile phase; the flow rate was 1.0 mL·min-1; the detection wave was set at 207 nm and the column temperature was kept at 30 ℃. Result： The best extraction technology of conduritol A was ultrasonic extraction in 50% methanol of 150 times for 50 min.The liner range of conduritol A was 0.400 4-20.02 μg（r=0.999 7）; the average recovery rate was 98.98%, RSD was 1.15%（n=6）. Conclusion： The method was accurate, quick, sensitive with good reproducibility, it could be used as the quality control method of the flower of Gymnema sylvestre.

关 键 词：匙羹藤 牛弥菜醇A 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]