加味交泰丸对大鼠糖尿病视网膜病变的防治作用及其机制  

Preventive and Therapeutic Effect of Jiawei Jiaotai Pill on Diabetic Retinopathy in Diabetic Rats and its Potential Mechanism

作　　者：陈广[1] 陆付耳[2] 杨华静[3] 董慧[2] 徐丽君[2] 邹欣[2] 黄召谊[1] 王开富[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合科,武汉430030 [2]华中科技大学同济医学院附属同济医院中西医结合研究所,武汉430030 [3]华中科技大学同济医学院附属同济医院眼科,武汉430030

出　　处：《中国实验方剂学杂志》2013年第6期183-187,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30801492)

摘　　要：目的:探讨加味交泰丸对大鼠糖尿病视网膜病变的防治作用及其作用机制。方法:将大鼠随机分为正常组、造模组,造模组大鼠采用小剂量链脲佐菌素(streptozotocin,STZ,30 mg.kg-1)尾静脉注射加高脂饲料喂养的方法建立2型糖尿病模型,2周后筛选糖耐量异常者随机分为糖尿病模型组和治疗组,治疗组以加味交泰丸煎剂2.05 g.kg-1ig,干预100 d后分别进行口服葡萄糖耐量试验(OGTT),检测糖化血红蛋白(HbA1C)及氧化应激相关指标。观察视网膜超微结构和神经节细胞的凋亡情况。结果:与正常组比较,模型组大鼠OGTT异常(P<0.01),HbA1C(P<0.01)及氧化相关指标活性升高、抗氧化相关指标活性下降(P<0.01),视网膜毛细血管基底膜明显增厚、神经节细胞凋亡增多;与模型组比较,治疗组大鼠OGTT改善(P<0.01或P<0.05),HbA1C(P<0.01)及氧化相关指标活性下降、抗氧化相关指标活性升高(P<0.01),视网膜毛细血管基底膜未见明显增厚、神经节细胞凋亡减少。结论:加味交泰丸可防治大鼠早期糖尿病视网膜病变,可能与其抗氧化应激及减少视网膜神经节细胞凋亡有关。Objective： To observe the preventive and therapeutic effect of Jiawei Jiaotai pill on rats diabetic retinopathy and its potential mechanism. Method： The model of type 2 diabetes mellitus in rats was established by injecting streptozotocin from tail vein and feeding with high fat and high caloric diet.The rats were randomly divided into normal group, model group and Jiawei Jiaotai pill group.Rats in Jiawei Jiaotai pill group were treated by Jiawei Jiaotai pill decoction from gastric tube.The oral glucose tolerance test（OGTT）, hemoglobin （HbA1C） and the relative parameters of oxidative stress in the serum were determined after 100 days.Ultrastructural changes in the rat retina were examined under the electron microscop.The apoptosis indexe of the ganglion cells was determined with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling（TUNEL） method and the results were analyzed in a computer-picture system. Result： The fasting blood glucose, postpradial blood glucose at 1 hour（BG-1h）, postpradial blood glucose at 2 hour（BG-2h）, HbA1C and malonaldehyde （MDA） level were all increased in model group while significantly decreased nitrogen monoxide（NO）, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activity in serum compared with those in normal group.The thickness of retinal capillary basement membrane was increased in the rats of model group.The apoptosis indexe of the ganglion cells in rats of model group was apparently higher than that in rats of normal group.Compared with those in model group, FBG, BG-1h, BG-2h, HbA1C and MDA level were all decreased in rats of group treated with Jiawei Jiaotai pill while significantly increased NO, SOD and GSH-Px activity in serum.The thickness of retinal capillary basement membrane was decreased in the rats treated with Jiawei Jiaotai pill.The apoptosis indexes of the ganglion cells in rats of treated group was apparently lower than that in rats of model group. Conclusion： Jiawei Jiaotai pill c

关 键 词：加味交泰丸 糖尿病视网膜病变 氧化应激 视网膜神经节细胞 

分 类 号：R285.5[医药卫生—中药学]