地黄不同炮制品组成的增液汤降低糖尿病大鼠血糖血脂作用的对比研究  

Comparison in treatment of Hypoglycemia and Hyperlipemia by Zengye Tang Prescription with Rehmanniae Radix Processed by Different Method in Diabetes Rats

作　　者：段卫娜[1] 张振凌[1] 孔莹莹[1] 陈智钦[1] 李敏兰[1] 

机构地区：[1]河南中医学院,郑州450046

出　　处：《中国实验方剂学杂志》2013年第6期187-191,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药行业科研专项(20110700701)

摘　　要：目的:通过对地黄不同炮制品组成的增液汤降低糖尿病模型大鼠血糖、血脂作用的对比研究,说明生地黄与熟地黄功效异同。方法:SD雄性大鼠高脂肪饲料饲养,一次性腹腔注射(ip)链脲佐菌素(STZ)35 mg.kg-1建立糖尿病模型;按血糖随机分为模型对照组,阳性盐酸二甲双胍组(1.5 g.kg-1),鲜地黄-增液汤组(10.2 g.kg-1),生地黄-增液汤水提组(7.8 g.kg-1),熟地黄-增液汤水提组(7.8 g.kg-1),生地黄-增液汤醇提组(7.8 g.kg-1),熟地黄-增液汤醇提组(7.8 g.kg-1),每组10只,连续灌胃(ig)给药3周,分别观察各组大鼠体重、摄食量、饮水量及血糖血脂的变化。结果:与对照组相比,鲜地黄-增液汤组血糖(9.22±0.88)mmol.L-1和生地黄-增液汤水提组血糖(7.65±1.02)mmol.L-1、醇提组血糖(8.35±2.58)mmol.L-1与模型组血糖(12.72±1.28)mmol.L-1相比均能极显著降低(P<0.01),同时均能极显著降低糖尿病大鼠摄食量、饮水量、口服糖耐量和血清甘油三酯(TG)(P<0.01),生地黄-增液汤水提组还能极显著性降低血清总胆固醇(CHO)(2.12±0.33)mmol.L-1(P<0.01)和显著性升高高密度脂蛋白(HDLC)(1.07±0.21)mmol.L-1,鲜地黄-增液汤和生地黄-增液汤醇提组能显著性降低CHO(P<0.05),对HDLC则无显著性差异,熟地黄-增液汤水提、醇提组对血糖和血脂的影响与模型组相比均无显著性差异。结论:鲜地黄、生地黄组成的增液汤都有降低糖尿病大鼠的血糖和血脂的作用,且水提物、醇提物无明显的差异性;熟地黄-增液汤水提、醇提物对血糖和血脂作用均不明显。地黄生熟异用对增液汤降糖、降脂作用影响较大。Objective： The comparison in treatment of hypoglycemia and hyperlipemia by Zengye Tang prescription with Rehmanniae Radix Processed by different method in diabetes rats was performe. Method： The high fat diet and intraperitoneal injection （ip） streptozotocin （STZ） 35 mg·kg-1 was used to establish diabetic model.Three weeks later ig, weight, food intake, the amount of water, hypoglycemic and hyperlipemia were observe. Result： Fresh Rehmanniae Radix, Rehmanniae Radix and Rehmanniae Radix Processed of Zengye Tang water extraction, alcohol extract could significantly reduce food intake, the amount of water, oral glucose tolerance and triglyceride（TG）, radix Rehmanniae Radix of Zengye Tang water extraction also could significantly reduce total cholesterol （CHO） and increase high density lipoprotein （HDLC）, fresh Rehmanniae Radix Radix and Rehmanniae Radix alcohol extract could reduced CHO, and there is no significant differencein HDLC, Rehmanniae Radix preparata water extraction, alcohol extract had no significant difference compared with model group. Conclusion： Fresh Rehmanniae Radix and Rehmanniae Radix could reduce the hyperglycemic and hyperlipemia in diabetes rats, water extraction and alcohol extract have no significant difference.Rehmanniae Radix preparata water extraction, alcohol extract have no apparent effect. Rehmanniae Radix with different processed method have a great influence on the hyperglycemic and hyperlipemia.

关 键 词：地黄炮制 增液汤 糖尿病 

分 类 号：R285.5[医药卫生—中药学]