复方乌骨藤胶囊对Lewis肺癌小鼠TNF-α,NK活性的影响  

Effect of Compound Wuguteng Capsule on TNF-α,NK activity in Mice with Lewis Lung Cancer

作　　者：张晓双[1,2] 孙建宁[1] 宋延平[3] 张恩户[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]陕西中医学院,陕西咸阳712046 [3]陕西省中医药研究院,西安710003

出　　处：《中国实验方剂学杂志》2013年第6期220-223,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察复方乌骨藤胶囊对Lewis肺癌小鼠肿瘤坏死因子-α(TNF-α)和自然杀伤细胞(NK)活性的影响。方法:取C57 BL/6雄性小鼠50只,常规接种Lewis肺癌,24 h内随机分为5组:荷瘤对照组、环磷酰胺组(CTX,0.02 g.kg-1)和复方乌骨藤胶囊高、中、低剂量组(2.25,1.125,0.563 g.kg-1)。每天ig给药1次,连续给药12 d。末次给药后次日,处死小鼠,剥离肿瘤,称重后计算抑瘤率,取脾脏制备脾细胞悬液,检测TNF-α,NK的活性。结果:复方乌骨藤胶囊高、中剂量组抑瘤率分别达到31.43%,22.38%;与荷瘤对照组TNF-α细胞毒指数(45.79±7.01)%相比,复方乌骨藤胶囊2.25,1.125 g.kg-1组的TNF-α细胞毒指数(60.48%±8.29)%,(56.00±9.72)%,显著提高(P<0.005,P<0.01);与荷瘤对照组NK细胞杀伤率(10.37±21.33)%相比,复方乌骨藤胶囊高、中、低剂量组的NK细胞杀伤率(32.23±24.03)%,(35.22±12.67)%,(26.70±12.11)%,显著提高(P<0.05,P<0.01);结论:复方乌骨藤胶囊对Lewis肺癌小鼠具有抑瘤作用,并能够增强其TNF-α和NK细胞活性,从而提高荷瘤小鼠的免疫功能。Objective： To study the effect of compound Wuguteng capsule on tumor necrosis factor-α（TNF-α）, natural killer cell （NK） activity of in mice with Lewis lung cancer. Method： Fifty C57 BL/6 male mice of vaccination Lewis lung cancer were randomly divided into five groups within 24 hours, tumor-bearing control group, Cyclophosphamide group （CTX, 0.02 g·kg-1）, Compound Wuguteng capsule large, medium and small dose groups （2.25, 1.125, 0.562 5 g·kg-1）.give medicine, once a day, for 12 days, the next day after the last time given medicine, put to death mice, strip and weigh the neoplasm, calculate the tumor inhibition rate, take the spleen preparation splenic cell suspension liquid, detection of TNF-α, NK activity. Result： Tumor inhibiting rate of compound Wuguteng capsule 2.25, 1.125 g·kg-1 dose group was 31.43% and 22.38%. Compared with tumor-bearing control group, TNF-α cytotoxic index （45.79±7.01）%, compound Wuguteng capsule 2.25, 1.125 g·kg-1 dose group of cytotoxic index（60.48%±8.29） %, （ 56.00±9.72）%, are significantly improved （P〈0.01, P〈0.05）.Compared with tumor-bearing control group, NK cell kill rate（10.37±21.33）%, compound Wuguteng capsule 2.25, 1.125, 0.562 5 g·kg-1 dose group of NK cell kill rate（32.23±24.03）%, （35.22±12.67）%, （26.70±12.11）%, are significantly improved （P〈0.01, P〈0.05）. Conclusion： Compound Wuguteng capsule has inhibitory effecton on tumor in mice with Lewis lung cancer, enhance TNF-α and NK activity so as to improve the immune function of mice tumor-burdened.

关 键 词：复方乌骨藤胶囊 抑瘤率 肿瘤坏死因子-Α 自然杀伤细胞 

分 类 号：R285.5[医药卫生—中药学]