六君祛痰解毒汤及其拆方对C57BL/6J小鼠Lewis肺癌转移的抑制作用  

Study of the Anti-metastasis Effect and Mechanism of Liujun Qutan Jiedu Decoction and its Functional Groups on Lewis Lung Metastasis Model in C_(57)BL/6J Mice

作　　者：陈华圣[1,2] 许爱华[1] 沈婷婷[1] 崔德利[1] 朱亚林 

机构地区：[1]扬州大学医学院,江苏扬州225001 [2]扬州大学临床医学院,江苏扬州225001

出　　处：《中国实验方剂学杂志》2013年第6期249-253,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省高校自然科学基础研究项目(06KJB360131)

摘　　要：目的:研究六君祛痰解毒汤及其拆方对小鼠Lewis肺癌转移的抑制作用及作用机制。方法:建立C57BL/6J小鼠Lewis肺癌转移模型,随机分为对照组、替加氟阳性对照组(78 mg.kg-1)、六君祛痰解毒汤(21 g.kg-1)及拆方六君祛痰汤(11 g.kg-1)、解毒汤(10 g.kg-1)和祛痰汤(5 g.kg-1)6组。ig给药15 d后剥瘤称取瘤质量,计算抑瘤率;摘取肺组织,固定后于解剖显微镜下观察肺表面转移灶数,计算肺癌转移率和抗转移率;用免疫组化和图像分析方法检测瘤组织细胞中CD44的表达;用放射免疫法检测血清中透明质酸(HA)及Ⅳ型胶原(ColⅣ)的含量。结果:各治疗组皆可抑制小鼠Lewis肺癌移植瘤的生长,肺癌转移灶明显减少,以六君祛痰解毒汤效果最好。给药各组皆程度不同地抑制小鼠移植瘤细胞CD44的表达,与模型对照组比较,六君祛痰解毒汤具有显著性差异(P<0.05),六君祛痰汤及祛痰汤具有极显著性差异(P<0.01);4个治疗组皆能降低荷瘤小鼠血清HA和ColⅣ的含量,六君祛痰汤的效果最好,六君祛痰解毒汤和祛痰汤次之(P<0.01),而解毒汤的作用则相对较弱。结论:六君祛痰解毒汤及其拆方对Lewis肺癌转移模型小鼠具有抗肿瘤转移作用,其机制可能与抑制肿瘤细胞对细胞外基质(ECM)的破坏、使其血清中HA和ColⅣ水平降低,下调肿瘤细胞CD44的表达、降低其与基质的黏附,进而抑制肿瘤细胞的运动、侵袭和转移有关。Objective： To study the anti-metastasis effect and mechanism of Liujun Qutan Jiedu decoction and its functional groups in metastasis of C57 BL/6J mice Lewis lung cancer. Method： The Lewis lung metastasis model was established in C57 BL/6J mice, randomly divided into 6 groups： saline model group, Tegafur positive control group （78 mg·kg-1）, Liujun Qutan Jiedu decoction （21 g·kg-1）, Liujun Qutan decoction （11 g·kg-1）, Jiedu decoction （10 g·kg-1）and Qutan decoction （5 g·kg-1）.After oral administration for 16 days, the tumor was stripped and weighed to calculate the inhibition rate.The metastatic tumor foci on lung surface in mice were counted to calculate the rate of cancerometastasis and anti-metastasis.Expression of CD44 in tumor cells was measured by immunohistochemistry and image analysis system, the concentrations of serum hyaluronic acid（HA） and collagen Ⅳ（Col Ⅳ） were detected by radioimmunoassay. Result： Liujun Qutan Jiedu decoction and its functional groups could inhibit the metastasis of Lewis lung carcinoma.The metastatic foci occurrence rates of treatment groups on lung surface were lower than model group.The effect of Liujun Qutan Jiedu decoction was the best.All treatment groups can inhibit the expression of CD44 to some degree.Compared with the model group, Liujun Qutan Jiedu decoction had a significant difference（P〈0.05）, Liujun Qutan decoction and Qutan decoction had a more significant difference（P〈0.01）;All four treatment groups could reduce the concentrations of serum HA and Col Ⅳ, however, the effect of Liujun Qutan decoction was the best, the effect of Liujun Qutan Jiedu decoction and Qutan decoction were the second（P〈0.01）, and the function of Jiedu decoction was relatively weak. Conclusion： Liujun Qutan Jiedu decoction and its functional groups all have anti-metastasis activities on Lewis lung metastasis model in C57BL/6J mice, and the underlying mechanisms were possibly related to inhibiting the breaking of extracellular matrix

关 键 词：六君祛痰解毒汤 LEWIS肺癌 CD44 透明质酸 Ⅳ型胶原 

分 类 号：R285.5[医药卫生—中药学]