人参皂苷compound K对慢性粒细胞白血病K562细胞增殖抑制和凋亡诱导作用  

Effect of ginsenoside compound K on the proliferation and apoptosis in K562 cells

作　　者：蒋传命[1] 何邵波[1] 周雨[1] 杨秦[1] 黄泽智[1] 

机构地区：[1]邵阳医学高等专科学校,湖南邵阳422000

出　　处：《中南药学》2013年第2期102-105,共4页Central South Pharmacy

基　　金：湖南省教育厅科学研究项目基金(编号:11C1159)

摘　　要：目的探讨人参皂苷compound K(CK)对慢性粒细胞白血病K562细胞增殖抑制和凋亡的影响。方法应用MTT法检测CK对K562细胞增殖的影响,用流式细胞仪分析细胞周期,DNA Ladder实验观察CK对K562细胞凋亡的影响。结果人参皂苷CK能显著抑制K562细胞的增殖,呈浓度依赖性,细胞阻滞于G2/M期;CK能诱导K562细胞的凋亡,呈浓度依赖性。结论人参皂苷CK具有抑制K562细胞增殖和诱导凋亡作用。Objective To investigate the effect of ginsenoside CK on the proliferation and apoptosis in K562 cells. Methods Effects of ginsenoside CK on the K562 cell proliferation and apoptosis were determined by MTT assay and DNA fragmentation assays. K562 cell cycle and apoptotic percentage were detected by flow cytometry. Results Ginsenoside CK significantly inhibited the proliferation of K562 cells in a dose-dependent manner. In addition, apoptosis of K562 cells was induced by ginsenoside CK in both dose-dependent and time-dependent manners. Apoptosis-characteristic DNA ladders were observed by DNA ladder assays. Conelusion Ginsenoside CK plays a crucially important role in exerting its anti-leukemic effect via inhibiting the proliferation and inducing apoptosis in K562 cells.

关 键 词：人参皂苷CK K562细胞 细胞增殖 细胞凋亡 

分 类 号：R965[医药卫生—药理学]