高效液相色谱法测定地锦草中鞣花酸的含量  

Determination of ellagic acid in Herba Euphorbiae by HPLC

作　　者：黄彩虹[1,2] 刘海涛[1,2] 雷鹏[1,2] 尹桃[1,2] 蓝珊娜[3] 

机构地区：[1]中南大学湘雅医院,长沙410008 [2]中南大学药学院,长沙410013 [3]桂林医学院,广西桂林541004

出　　处：《中南药学》2013年第2期136-138,共3页Central South Pharmacy

基　　金：湖南省中医药科技基金重点课题(编号:20090008);长沙市科技局重点项目(编号:K0802115-31)

摘　　要：目的建立地锦草中鞣花酸含量的测定方法。方法采用高效液相色谱法,色谱柱:OdyssilC18(4.6 mm×250 mm,5μm);流动相:甲醇-0.1%磷酸(40:60);流速:1.0 mL min-1;检测波长:254 nm;柱温:30℃。结果鞣花酸进样量在0.032～0.476μg与峰面积呈良好的线性关系,r=0.999 9,平均加样回收率为97.3%,RSD为1.4%。结论该方法简便准确,重现性好,可作为地锦草中鞣花酸的含量测定方法。Objective To establish an HPLC method to determine ellagic acid in Herba Euphorbiae. Methods The HPLC system consisted of Odysssil C18 （4.6 mm × 250 mm, 5μm） and methanol-0.1%H3PO4 （40 ： 60） as the mobile phase at 1.0 mL·min^-1. The detection wavelength was 254 nm and the column temperature was 30 ℃ . Results The calibration curve showed a good linear relationship at 0.032 -- 0.476 μg, r = 0.999 9, the average recovery was 97.3% and RSD was 1.4%. Conclusion The method is simple, accurate and repeatable, which can be used for the determination of ellagic acidin in Herba Euphorbiae.

关 键 词：地锦草 鞣花酸 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学] R927.2[医药卫生—药学]