福建省一起流行性腮腺炎爆发的病毒分离及基因特征分析  

Genetic Characterization of the Mumps Virus Isolated from An Outbreak in Fujian

作　　者：杨秀惠[1] 管辉日 严延生[1] 何爱华[1] 张红榕[1] 许江阳[1] 

机构地区：[1]福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福州350001 [2]永安市疾病预防控制中心,福建永安366000

出　　处：《中国疫苗和免疫》2013年第1期43-46,共4页Chinese Journal of Vaccines and Immunization

摘　　要：目的对福建省一起流行性腮腺炎(腮腺炎)爆发疫情进行病毒分离,了解其基因特征。方法应用非洲绿猴肾细胞分离病毒,逆转录-聚合酶链反应扩增小疏水蛋白(Small Hydrophobic Protein,SH)基因片段,测序并分析其基因特征。结果从爆发点采集的4例标本中,分离到3株腮腺炎病毒,均属于G基因型。此福建株与国内其他省流行的F基因型病毒株核苷酸差异性>9%,与S(上海,Shanghai)79疫苗株SH基因核苷酸差异17.6%,与1999～2005年英国流行的G2基因亚型病毒株核苷酸同源性最高(97.4%～99.8%)。结论此起爆发为G基因型腮腺炎病毒引起,是否为输入性病毒?目前在中国使用的疫苗所产生的抗体能否完全阻断?需进一步加强监测。Objective To genetically characterize mumps viruses associated with a mump outbreak in Fujan province in 2011. Methods Viruses were isolated using veto cells. Small hydrophobic （SH） gene were amplified by reverse transcription-polymerase chain reaction （RT-PCR）, sequenced and analyzed along with reference strain sequences downloaded from genbank. Results Of 4 throat swabs collected from the outbreak, 3 mumps virus strains were isolated, and identified as G genotype. The isolated strains showed that more than 9% sequence divergence from predominant F genotype strains circulating in other provinces of China, 17.6% sequence divergence from S （Shanghai）79 vaccine strain, 97.4%-99.8% sequence homology with the G2 subgenotype strains isolated from the United Kingdom between 1999 and 2005. Conclusion The outbreak was caused by mumps virus of G genotype. Further surveillance is needed to clarify whether the virus was imported, and whether its transmission can be completely blocked by the vaccine in China.

关 键 词：流行性腮腺炎病毒 基因型 分子流行病学 

分 类 号：R512.11[医药卫生—内科学]