DNA的荧光分析——DNA对吖啶橙(AO)—藏红T(ST)能量转移影响的研究  

Fluorescent Analysis of DNA-Research of DNA on Acridine orange (AO)-Safranine (ST) Energy Troursfer

作　　者：陈鸿琪[1] 

机构地区：[1]山东临沂师范学院化学系,山东 临沂 276001

出　　处：《新疆大学学报（自然科学版）》2000年第4期33-39,共7页Journal of Xinjiang University(Natural Science Edition)

摘　　要：介绍了近几年荧光能量转移体系用于 DNA分析测定方法的进展 .通过实验对吖啶橙( AO)—藏红 T( ST)荧光体系能量转移进行分析 ,说明使用该本系为荧光探针对 DNA定量测定的可行性 .然后通过 Forster能量转移理论 ,计算 DNA加入前后 AO- ST体系临界距离变化 ,从某种程度上说明 DNA对 AO- ST荧光淬灭的影响 .This paper introduces recent progress in method of applying fluorescence energy transfer system to the analytic test of DNA,the energy transfer of Acridine orange (AO) Safranine (ST) System is analyzed through experiments,proving the possibility of quantatively testing DNA with the fluorescene probe of this system,critical ranges of AO-ST before and after the addition of DNA are calculated respectively, according to Forster theory of energy transfer,thus to some extent illustrating the influence of DNA on AO ST fluoresence quenching

关 键 词：能量转移 吖啶橙 藏红 DNA 荧光分析 

分 类 号：Q523[生物学—生物化学]