7种石斛多糖质量分数的测定及单糖组成分析  

Determination of the content and monosaccharide composition of 7 species of Dendrobium polysacchrides

作　　者：龚庆芳[1] 周浩[1] 王新桂[1] 何金祥[1] 付传明[1] 郭伦发[1] 

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,桂林541006

出　　处：《食品科技》2013年第3期172-175,共4页Food Science and Technology

基　　金：广西科技攻关项目(桂科攻10100012-5);广西林业厅项目(桂林科字[2011]第07号;桂林科字[2012]第21号);桂林市科技攻关项目(20110107-1);广西植物研究所基本业务费项目(桂植业12008)

摘　　要：通过采用苯酚-硫酸法测定多糖质量分数、柱前衍生化HPLC法测定多糖中的单糖组成的方法对铁皮石斛、重唇石斛、钩状石斛、细茎石斛、金钗石斛、鼓槌石斛、河南石斛进行检测。研究结果表明:铁皮石斛、重唇石斛、钩状石斛、细茎石斛、金钗石斛、鼓槌石斛、河南石斛的多糖质量分数为5.04%～27.33%,其中铁皮石斛多糖含量最高,它们的多糖均为含有D-甘露糖、葡萄糖、半乳糖、阿拉伯糖的杂多糖。这7种石斛多糖中的D-甘露糖、葡萄糖、半乳糖、阿拉伯糖的摩尔比存在差异性。The content of polysaccharides and the monosacchride composition of polysacchrides from Dendrobium were studied by phenol-sulfuric acid method and pre-column derivatization HPLC. The results showed that the content of polysacchrides in the genus Dendrobium ranged from 5.04% to 27.33%, of which the polysacchrides content of Dendrobium officinal was the highest. The polysacchrides of D.officinale Kimkuraet Migo, D.hercoglossum Rchb.f, D.aduncum Wall.ex Lindl, D.Moniliforme, D.nobile Lindl, D.chrysotoxum and D.henanense J. L. Lu et L.X.Gao were composed of D-Man, GIc, Gal and Arab, but the content of its are singnificantly different.

关 键 词：石斛 多糖 柱前衍生化 单糖 

分 类 号：R284.1[医药卫生—中药学]