冷却肉中糖原的生物传感器检测  

Determination of glycogen content in chilled meat by biosensor

作　　者：冯东[1] 李雪梅[1] 王丙莲[1] 李大海[1] 

机构地区：[1]山东省科学院生物研究所山东省生物传感器重点实验室,济南250014

出　　处：《食品科技》2013年第3期252-255,共4页Food Science and Technology

基　　金：济南市青年科技明星计划项目(20080121);山东省博士后创新基金项目

摘　　要：研究酶电极生物传感器法测定冷却肉中的糖原含量。利用淀粉葡萄糖苷酶将糖原分解为葡萄糖,再用葡萄糖氧化酶电极生物传感器测定葡萄糖含量。结果表明:生物传感器在葡萄糖浓度0.5～20mg/mL范围内线性关系良好(R=0.9998);加样回收率为98.8%～101.2%,抗干扰性强;对肌糖原的检测与蒽酮比色法、酶解分析法相比较,差异不显著(P>0.05);且仪器重现性和稳定性较好。葡萄糖生物传感器用于肌糖原含量测定具有检测精度高、速度快、稳定性好、使用成本低等优势,有望成为检测冷却肉中肌糖原含量的新方法、新手段。The enzyme electrode biosensor method for glycogen detection was researched. The glycogen was decomposed to glucose by starch glucosidase, and then glucose was determined by glucose oxidase electrode biosensor. The method showed good lineadty, linear range was from 0.5 mg/mL to 20 mg/mL （the correlation coefficient R was 0.9999）. Using this method, different extracts were measured and the average recoveries range from 98.8% to 101.2%. It proved to be a very useful method for testing large quantities of samples. Compared with the known methods, such as anthrone colorimetry and enzymatic analysis, this method is simple and rapid.

关 键 词：糖原 生物传感器 酶电极 葡萄糖氧化酶 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]