高梁根质膜K^(+)转运蛋白的提取和纯化  

Extraction and purification of K^(+)transport protein from plasma membrane of sorghum roots

作　　者：乙引[1] 汤章城[2] 倪晋山 姜伯尼 杨浩舒 

机构地区：[1]贵州师范大学生物科学技术系,贵州 贵阳 500001 [2]中国科学院上海植物生理研究所,上海 200032 [3]贵阳市环境保护局，贵州 贵阳 550002

出　　处：《贵州师范大学学报（自然科学版）》2000年第4期28-30,共3页Journal of Guizhou Normal University：Natural Sciences

基　　金：国家自然科学基金重点资助项目(3920040)

摘　　要：采用蔗糖密度梯度法提取高梁根质膜 ,并以DextranT - 5 0 0和PEG - 335 0G两相法纯化质膜 ,用6 5mmol/LCHAPS增溶膜蛋白 ,然后 ,经Superose 12柱和MonoQ柱进行FPLC纯化 ,最终收集到具有K+转运功能的蛋白质。经SDS -PAGE分析表明 ,它至少含有一条This paper describes partial purification and characterization of a vandate-sensitive K +-ATPase from PM of Sorghum vargular. Purification is based on the following procedure: extraction of membrane with CHAPS, followed by fraction of the extract by successive FPLC-mediated gel permeation and ion-exchanes chromatography. The overall yield was 5%. The partially purfied ATPase appares to contain a polypeptide component of Mr=80kd.

关 键 词：转运蛋白 纯化 提取 高梁根质膜 

分 类 号：Q946.1[生物学—植物学]