毒死蜱主要代谢物3,5,6-TCP在土壤、植物中的累积与转化  

Accumulation and transformation of the chlorpyrifos metabolite 3,5,6-TCP in soil and plants

作　　者：孙宝利[1] 陕红[1] 袁志华[2] 曾希柏[1] 

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,农业部农业环境与气候变化重点开放实验室,北京100081 [2]农业部环境保护科研监测所,天津300191

出　　处：《河北农业大学学报》2013年第1期85-89,共5页Journal of Hebei Agricultural University

基　　金：科技支撑项目(2012BAD14B02);国家自然科学基金项目(41201234)资助

摘　　要：本研究应用盆栽试验和室内土壤培养试验,考察毒死蜱主要代谢物3,5,6-三氯-2-吡啶(简称3,5,6-TCP)在植物、土壤中的累积与转化,研究毒死蜱代谢产物累积量变过程及其影响因素。结果表明:(1)推荐剂量喷施毒死蜱的第7天和第14天,3,5,6-TCP在植物体和土壤中的累积量达到峰值,直到20d后,植物体内几乎测不出3,5,6-TCP残留,而土壤中3,5,6-TCP残留量仍较高,其降解速度较慢。(2)3,5,6-TCP在5种土壤中的累积量主要与土壤自身物理性质有关,并随着温度和施用量的增加而增加,与施肥量和湿度相关不大,5种土壤中3,5,6-TCP的残留累积量在0.14～0.21μg/g。(3)在厌氧条件下培养土壤样品,应用色谱保留时间验证3,5,6-TCP在土壤中能继续转化生成3,5,6-三氯-2甲氧基吡啶(TMP),表明毒死蜱代谢转化途径与微生物量及种类有关,使得毒死蜱的安全降解具有可行性。By pot experiment and laboratory soil incubation experiment, the accumulation and trans- formation of 3,5,6-trichloro-2-pyridinol（3,5,6-TCP）, the main metabolite of chlorpyrifos in plant and soil were monitored in order to investigate the accumulation process and its affecting factors of the me- tabolite of chlorpyrifos in plants and soils. The results showed that： （1）The accumulative amount of 3, 5,6-TCP in plant and soil reached the peak at the 7th day and 14th day, respectively, after chlorpyri- los applied by recommended dose; the degradation rate of 3,5,6-TCP in plant was faster than that in plant and the residue amount of 3,5,6-TCP in plant was undetectable 20 days later; （2） Physical properties of 5 kinds of soil has the decisive effects on the accumulated residue of 3,5,6-TCP and the accumulated value increased with the increasing of the application amount of chlorpyrifos and tempera- ture, however, there were no relationship with the amount of fertilizer and humidity. The average accumulated value of 3,5,6-TCP in five kinds of soil was among 0. 14-0.21gg/g; （3）by continuous monitoring sealed soil sample and using characteristics of chromatographic retention time, it further proved that 3,5,6-TCP was the metabolite of chlorpyrifos and could further transform into 3,5,6-trichloro-2-methoxypyridin （TMP）. The result also showed that the degradation way of chlorpyrifos was related to the microbial and its species which can make the safe degra- dation of chlorpyrifos possible.

关 键 词：毒死蜱 3 5 6-TCP 累积 转化 

分 类 号：S767.3[农业科学—森林保护学]