Wnt10b对体外培养的人毛乳头细胞诱导毛囊生成能力的影响  

The effects of wnt10b on the long-term cultured human dermal papilla cells in vitro

作　　者：张志丹[1] 胡志奇[1] 李科成[1] 冯传波[1] 肖顺娥[1] 苗勇[1] 

机构地区：[1]南方医科大学南方医院整形外科,广州市510515

出　　处：《实用医学杂志》2013年第5期688-691,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:31170949);广州市科技计划项目(编号:2010Y1-C201);省部产学研结合项目(编号:2011B090400014)

摘　　要：目的:探讨Wnt10b对体外长期培养的人毛乳头细胞诱导毛囊生成能力的影响作用。方法:将人毛乳头细胞在体外传代培养到第8代后,加入人重组蛋白Wnt10b共培养,通过免疫荧光染色检测人毛乳头细胞的β-catenin表达,Real-time QPCR检测与毛乳头细胞诱导能力相关的特异性标记物碱性磷酸酶(ALP)和胰岛素样生长因子-1(IGF-1)的表达,以及ALP染色检测Wnt10b对人毛乳头细胞的作用。结果:Wnt10b能够通过经典的Wnt/β-catenin信号通路作用于体外长期培养的人毛乳头细胞,上调人毛乳头细胞特异性标记物ALP和IGF-1的表达,从而维持人毛乳头细胞的诱导能力。结论:Wnt10b能够通过上调特异性标记物ALP和IGF-1的表达来维持体外长期培养的人毛乳头细胞的毛囊诱导能力。Objective To investigate the effects of Wnt10b on the hair follicle induction of long-term cultured human dermal papilla cells （HDPCs） in vitro. Methods The HDPCs of generation ability 8th were used for experiments. The recombinant human WntlOb （1 μg/mL） was added to the medium and cultured with HDPCs. To evaluate the effects of WntlOb treatment, β-eatenin expression and alkaline phosphatase （ALP） activity of the HDPCs were examed, and the gene expression of HDPCs markers were determined by Real-time quantitative PCR. Results WntlOb has a significantly effects on the HDPCs through Wnt/β-catenin signaling pathway, it can successfully upregulated the specific expression of HDPCs hair follicle induction marker molecules, such as ALP and insulin-like growth factor-1 （IGF-1）. Conclusion WntlOb can maintain the hiar follicle-inducing ability of the long-term cultured HDPCs through the classic canonical Wnt/β-catenin signaling pathway in vitro.

关 键 词：Wnt10b Β-连环蛋白 胰岛素样生长因子-1 人毛乳头细胞 碱性磷酸酶 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]