胎盘间充质干细胞治疗大鼠缺氧缺血性脑病的实验研究  

Placenta-derived mesenchymal stem cells for hypoxic ischemic brain damage in rats

作　　者：丁红芳[1] 丁慧芳[2] 高欣义[1] 鞠秀丽[3] 伊心浩[2] 周明琪[1] 

机构地区：[1]胜利油田中心医院儿科,山东省东营市257034 [2]胜利油田中心医院血液科,山东省东营市257034 [3]山东大学齐鲁医院儿科,济南市250012

出　　处：《实用医学杂志》2013年第5期711-714,共4页The Journal of Practical Medicine

摘　　要：目的:探讨胎盘间充质干细胞(PDMSCs)治疗新生大鼠缺氧缺血性脑病(HIBD)的效果及其机制。方法:Wistar大鼠随机分为对照组、HIBD组、HIBD+PDMSCs组和HIBD+成纤维细胞组。移植后14d评估各组生长发育情况、神经功能,分别采用RT-PCR法、Western Blot法检测损伤侧海马组织的血红素加氧酶-1(HO-1)、核因子E2相关因子2(Nrf2)的基因和蛋白表达。结果:HIBD+PDMSCs组脑萎缩、神经行为障碍均改善,且体重显著升高,与对照组比较有明显差(P<0.05)。HIBD组HO-1和Nrf2的基因和蛋白表达均高于对照组(P<0.05),HIBD+PDMSCs组二者含量均较HIBD组明显增加(P<0.05),HIBD+成纤维细胞组的各项指标与HIBD+PDMSCs组相比均有统计学差异(P<0.05)。结论:PDMSCs移植能够改善新生HIBD大鼠的神经功能和发育,可能是通过部分上调Nrf2/ARE/HO-1通路发挥作用。Objective To investigate the effects of placenta-derived mesenchymal stem cells (PDMSCs) on hypoxic ischemic brain damage (HIBD) in rats and its mechanism. Methods Rats were randomized into control group, HIBD group, HIBD+PDMSCs group, and HIBD+fibroblasts group. After 2 weeks of treatment, the growth and nerve function were measured, and the expressions of HO-1 and Nrf2 gene and protein were detected by RT-PCR and Western Blot. Results As compared to those in control group, brain atrophy and neurological dysfunction were ameliorated and body weight was increased in HIBD+PDMSCs group (P < 0.05). The expressions of HO-1 and Nrf2 gene and protein were increased from control group to HIBD group, and to HIBD+PDMSCs group (all P < 0.05). All these index were significantly different between HIBD+fibroblasts group and HIBD+PDMSCs group (P < 0.05). Conclusion PDMSCs may play a critical role in the neuroprotection in rats with HIBD through up-regulating Nrf2/ARE/HO-1 pathway.

关 键 词：缺氧缺血 脑 间质干细胞 胎盘 氧化 大鼠 

分 类 号：R722.1[医药卫生—儿科]