亚低温对窒息大鼠心肺复苏后S100B蛋白及胶质纤维酸性蛋白表达的影响  

Effect of mild hypothermia on S100B and GFAP expression after cardiopulmonary resuscitation in anoxia-induced rats

作　　者：黄效模[1] 汤旭[1] 周厚荣[1] 张谦[1] 曾德珍[1] 周霞[1] 

机构地区：[1]贵州省人民医院急诊科,贵阳市550002

出　　处：《实用医学杂志》2013年第5期715-718,共4页The Journal of Practical Medicine

基　　金：贵州省科学技术厅资助课题(编号:黔科合SY[2010]3126号)

摘　　要：目的:探讨亚低温对窒息大鼠心肺复苏后血清、大脑皮层S100B蛋白及胶质纤维酸性蛋白(GFAP)表达的影响。方法:60只SD大鼠随机分为假手术组(A组)、常温组(B组,常规复苏)、保存性低温组(C组,常规复苏,心跳骤停时给予亚低温治疗)、复苏性低温组[D组,常规复苏,自主循环恢复(ROSC)后30min给予亚低温治疗],各组于心跳骤停时和ROSC后2、4h取血,处死大鼠取脑组织,观察各时点血清大脑皮层S100B蛋白、GFAP的动态变化。结果:(1)B、C、D各组血清S100B蛋白从ROSC后2h起开始升高,并持续上升至4h,与A组比较,各时点血清S100B蛋白含量及大脑皮层S100B蛋白表达均显著增高(P<0.05)。(2)与B组比较,C组ROSC后2h血清S100B蛋白含量降低,差异无统计学意义(P>0.05),ROSC后4h血清S100B蛋白含量显著降低(P<0.05),皮层S100B蛋白表达显著减少(P<0.05)。(3)与A组比较,B、C、D各组ROSC后4h大脑皮层GFAP表达均显著增加(P<0.05)。结论:大鼠血清、大脑皮层组织S100B蛋白和GFAP在ROSC后早期即有表达,可以作为诊断脑损伤及判断预后的敏感标志物;亚低温可减轻窒息大鼠心肺复苏后脑损伤。Objective To explore the effects of mild hypotherrnia on expression of S100B and GFAP in serum and brain after cardiac arrest （CA）-cardiopulmonary resuscitation （CPR） in anoxia-indueed rats. Methods Sixty adult SD rats were randomly divided into 4 groups： sham-operation group （group A）, nonnothermia group （group B, CPR）, instant hypothermia group （group C, used hypothermia on CA immediately）, and resuscitation hypothermia group [ group D, used hypothermia after 30 rain of restoration of spontaneous circulation （ROSC）]. Blood was taken at CA times, 2 h and 4 h after ROSC, respectively. S100B and GFAP expression were observed at each time point. Results The serum levels of S100B protein was increased significantly at 2 h after ROSC and continue increased at 4 h in group B, C and D. The expression of S100B protein in serum and cerebral cortex were increased significantly at each time point when compared with group A （P 〈 0.05 ）. The serum levels of S100B protein decreased at 2 h after ROSC in group C when compared with group B, but there was no significant difference between them （P 〉 0.05 ）. The expression of S100B protein in serum and cerebral cortex was decreased significantly at 4 h after ROSC when compared with group B （P 〈 0.05）. Compared to group B, the expression of GFAP protein in cerebral cortex was increased at 4 h after ROSC in group B, C and D （P 〈 0.05）. Conclusions The S100B and GFAP in serum and cerebral cortex were expressed at the early phase after ROSC in rats, which can be used as a sensitive marker for early diagnosis of cerebral injury and prognosis. Mild hypothermia could alleviate the cerebral injury in anoxia-induced rats after cardiopulmonary resuscitation.

关 键 词：心肺复苏术 亚低温 脑保护 SIOOB GFAP 

分 类 号：R459.7[医药卫生—急诊医学]