覆土中放线菌对暗褐网柄牛肝菌出菇的影响  

Effect of Actinomycetes of Casing on Fruiting of Phlebopus portentosus

作　　者：王文兵[1] 曹旸[1] 纪开萍[1] 刘静[1] 张春霞[1] 何明霞[1] 

机构地区：[1]云南省热带作物科学研究所,云南景洪666100

出　　处：《中国食用菌》2013年第2期36-38,共3页Edible Fungi of China

基　　金：云南省应用基础研究资助项目(2008CD193)

摘　　要：本试验对暗褐网柄牛肝菌人工栽培时覆土中的放线菌进行了分离纯化。通过回接覆土试验研究了覆土中放线菌对暗褐网柄牛肝菌子实体生长的影响;通过16S rDNA序列分析对有益放线菌菌株进行了初步鉴定。结果表明,分离得到的37株放线菌菌株中有12个菌株分别在牛肝菌菌丝生长、原基发生及子实体生长等方面表现出促进作用。16SrDNA序列分析结果显示,12株放线菌中11株属于链霉菌属,1株属于游动放线菌属。Actinomycetes were isolated from the casing layer which were used in the cultivation of Phlebopus portentosus. The effects of actinomycetes on fruiting of Phlebopus portentosus were studied, and beneficial strains were identified. The results indicated that 12 of total 37 actinomycete strains could promote the development of mycelium and fruit body of Phlebopus portentosus. Based on the 16S rD- NA sequences analysis, 11 of 12 beneficial strains were proposed as known species of genus Streptomyce and one was Actinoplanes.

关 键 词：暗褐网柄牛肝菌 覆土 有益放线菌 

分 类 号：S646.3[农业科学—蔬菜学]