8个牛品种的大理石花纹评分相关基因变异的微卫星DNA池分析  

Study on Marbling Score Related Genes Variations Using Microsatellite Markers and DNA Pooling in 8 Cattle Breeds

作　　者：乔冬雨[1] 孙志颖[2] 孙少华[1] 楚康康[1] 刘媛[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071000 [2]河北农业大学生命科学学院,河北保定071000

出　　处：《中国畜牧杂志》2013年第5期1-4,共4页Chinese Journal of Animal Science

摘　　要：本研究以8个牛品种为研究对象,利用与大理石花纹评分基因相关的7个微卫星位点结合DNA池分析技术,探讨微卫星DNA多态性与8个牛品种大理石花纹评分间的关系,并根据性状同质性原理预测品种组合,以加快生产出高端"雪花"牛肉。结果表明:日本和牛与荷斯坦牛、安格斯牛、渤海黑牛的相似性系数均超过0.8,而日本和牛与其他牛品种的的相似性系数均小于0.8。利用MEGA4软件采用邻接法进行聚类,利木赞牛与西门塔尔牛先聚合在一起,再与草原红牛聚合;渤海黑牛与鲁西黄牛2个地方良种聚合在一起,以上5个品种聚为第1大类。荷斯坦牛与日本和牛聚合,再与安格斯牛聚在一起,它们聚为第2大类。根据本研究结果和国外肉牛杂交生产实践,从牛肉大理石花纹性状的同质性出发,建议利用日本和牛、荷斯坦牛为亲本,杂交生产高档"雪花"牛肉,以解决我国的肉牛牛源短缺问题。In this study, we combined 7 microsatellite loci associated with marbling score in 8 cattle breeds （Holstein, Simmental, Red Steppe, Limousin, Luxi cattle, Bohai Black cattle, Japanese Black Cattle, Angus cattle） with DNA pooling analytical technique to study the relationship among the polymorphism of each microsatellite markers, varieties similarity coefficient and the genetic distances. In order to produce the snowflake beef, the cattle varieties were predicted based on the character homogenicity principle. The similarity coefficients of Japanese Black Cattle between Holstein, Angus and Bohai Black cattle were all greater than O. 8, but smaller than O. 8 between other breeds. The results of cluster analysis by MEGA4 software using the neighbor-jioning method, Limousin and Simmental was first clustered together, and then was clustered with Red Steppe; Bohai Black cattle was clustered with Luxi cattle, these 5 breeds were classified one group. Holstein cows was clustered with Japanese Black cattle, then was clustered with Angus, they were classified one group. According to our research and foreign cross combinations practice, meanwhile think from the character homogenicity of marble, in order to resolve the source shortage of beef in our country, we advise to put Japanese Black cattle, Angus, Holstein, Bohai Black cattle as parents to crossbreeding produce the best beef.

关 键 词：DNA混合池 微卫星标记 大理石花纹评分 杂交组合 牛 

分 类 号：S823.2[农业科学—畜牧学]