超声萃取-响应面法优化淡竹叶多糖提取方法研究  

Optimization of ultrasound-assisted extraction of polysaccharides from Lophantherum gracile brongn by response surface methodology

作　　者：张海容[1] 白娟[1] 魏增云 刘勇麟[1] 陈金娥[1] 王迎进[1] 

机构地区：[1]忻州师范学院生化分析技术研究所,山西忻州034000 [2]忻州职业技术学院,山西忻州034000

出　　处：《化学研究与应用》2013年第3期303-310,共8页Chemical Research and Application

基　　金：山西省教育厅国际交流项目(2011-63)资助;山西省高校科技开发项目(晋教科20101122)资助;忻州师范学院院级科研基金项目(2011-52)资助

摘　　要：利用超声辅助萃取—响应面法提取淡竹叶多糖工艺进行研究。在单因素试验基础上,采用五因素三水平响应面分析法,使用中心组合(Box-Benhnken)试验设计原理得到二次线性回归方程,以多糖产率为响应面值作响应面图,确定超声提取淡竹叶多糖最佳工艺条件为:功率200W,温度50℃,液料比35,超声时间30min,提取次数2次,多糖产率为3.42%。验证实验表明,所得模型方程能较好地预测实验结果,拟合度较好。The technology of uhrasound-assisted extraction of polysaccharides from Lophantherum gracile brongn leaves was investi- gated with response surface methodology. On the basis of a series of one-factor experiments, second linear regression equation was obtained with pelysaccharid yield as a response surface value, the optimum extraction conditions of polysaccharides from Lophan- therum gracile brongn were determined by the Box-Benhnken design with five factors and three levels,which were as follows ：200W of extraction power,3Omin of extraction time,50℃ of extraction temperature and 35：1 （ mL · g-1 ） of liquid-to-solid ratio, and the ex- tration efficiency of polysacchafides was up to 3.42% under the optimized conditions. The confirmative test showed that the estab- lished model can well predict experimental results, and its fitting degree was good.

关 键 词：淡竹叶 多糖 超声提取 响应面分析 

分 类 号：O658.2[理学—分析化学]