基于分子识别作用鉴别人参基因的电化学传感器  

The electrochemical sensor for DNA of P.ginseng detection based on the molecular recognition technique

作　　者：赵辉[1] 王晓云[2] 樊浩[2] 崔汉峰[2] 李强根[2] 

机构地区：[1]周口师范学院化学系,河南周口466001 [2]江西中医学院药学院,江西南昌330004

出　　处：《化学研究与应用》2013年第3期322-327,共6页Chemical Research and Application

基　　金：国家自然科学基金项目(21265007)资助;江西省自然科学基金项目(20122BAB215024)资助;周口师范学院应用化学重点学科提供部分资助

摘　　要：本文以人参ITS及518 s基因上的SNP位点为检测对象,利用分子识别作用构建了一种电化学传感器,成功地对人参、西洋参进行了品种鉴别。本文设计合成了一种双标记DNA探针(DLP),该探针的一端标记了4-4-二甲氨基苯基偶氮苯甲酸(dabcyl)作为客体分子,另一端标记了金纳米颗粒作为电化学杂交指示剂。同时使用α-CD/MCNTs/GCE电极作为工作电极。由于DLP的茎环结构,只有在DLP与目标DNA杂交后,DLP上的dabcyl分子进入修饰电极表面的α-CD空腔中,进而DLP被α-CD修饰电极捕获。并且,通过金纳米颗粒的AuCl4-的电化学还原电流信号。可灵敏检测4.6×10-10mol.L-1的目标DNA。To identify single nucleotide polymorphisms （SNP） sites in ITS and 5. 8 s gene of Panax species, the electrochemical sen- sor was constructed for the identification of P. g/nseng and P. quinquefolium based on the molecular recognition technique. A novel dually-labeled DNA probe（DLP） in a stem-loop structure was designed to label at one end with dabcyl ,working as a guest mole- cule, and its another end was labeled with an Au nanopartiele, working as an electrochemical tag to indicate the electrochemical sig- nal of hybridization occurrence. One ot-CD/MCNT/GC eleetrude was fabricated for the hybridizer capture and electrochemical signal transduetion. After hybridized with the target DNA, the target-binding DLP caused the DLP＇s loop-stem structure opened and then the dabcyl molecule was easily, entering the cavity of the α-CD modified electrode and resulting in that the DLP could be captured by the α-CD modified electrode and the capture efficiency was proportion with the concentration of the target DNA. Therefore, the target hybridization event can be sensitively transduced via detecting the electrochemical reduction current signal of AuCl4- of Au nanopartictes labeled at the DLP.

关 键 词：人参DNA 电化学传感器 双标记DNA探针 单核苷酸多态性 

分 类 号：O657.1[理学—分析化学]