漆酚-酪氨酸功能基树脂的合成及对多酚氧化酶的固定化  

Synthesis of Urushiol-Tyrosine Resin and Immobilization of Polyphenol Oxidases

作　　者：雷福厚 吴健[1] 史伯安[2] 谭志伟 

机构地区：[1]广西民族学院化学化工系,南宁530006 [2]湖北民族学院

出　　处：《应用化学》2000年第4期414-416,共3页Chinese Journal of Applied Chemistry

基　　金：广西民族学院 1999年重点资助!项目 (99HXX0 0 0 0 4)

摘　　要：The urushiol tyrosine resin(UTR) was synthesized from tyrosine and gasoline solution of urushiol by using anhydrous AlCl 3 as catalyst. Its structure has been studied by IR, UV and DTA. The UTR Cu was obtained by impregnation of UTR in Cu 2+ solution. The polyphenol oxidase obtained from banana was immobilized on UTR Cu complexes. The immobilized oxidase(UTR Cu En) had an activity of 1 1 A/(min·g), that is, 6 5 times greater than that of oxidase immobilized on active carbon. A possible structure of the UTR Cu immobilized enzyme is suggested.The urushiol tyrosine resin(UTR) was synthesized from tyrosine and gasoline solution of urushiol by using anhydrous AlCl 3 as catalyst. Its structure has been studied by IR, UV and DTA. The UTR Cu was obtained by impregnation of UTR in Cu 2+ solution. The polyphenol oxidase obtained from banana was immobilized on UTR Cu complexes. The immobilized oxidase(UTR Cu En) had an activity of 1 1 A/(min·g), that is, 6 5 times greater than that of oxidase immobilized on active carbon. A possible structure of the UTR Cu immobilized enzyme is suggested.

关 键 词：漆酚-酷氨酸树脂 合成 多酚氧化酶 固定化 

分 类 号：Q814.2[生物学—生物工程]