虎杖中白藜芦醇和白藜芦醇甙的高速逆流色谱分离提纯及其分析  

Separation and Analysis of Resveratrol and Piceid in Polygonum Cuspidatum Sieb. et Zicc.

作　　者：陈雷[1] 杨福全[2] 张天佑[2] 韩雅珊[1] 陈尚武[1] 

机构地区：[1]中国农业大学食品学院,北京100094 [2]北京市新技术应用研究所,北京100035

出　　处：《分析测试学报》2000年第4期60-62,共3页Journal of Instrumental Analysis

摘　　要：采用高速逆流色谱技术分离提纯中药虎杖中的活性多酚成分白藜芦醇和白藜芦醇甙。确定了样品前处理方法 ,选择了逆流色谱流动相体系 ,经高效液相色谱检验纯度 ,1HNMR及质谱判定结构 ,证实所得样品为白藜芦醇和白藜芦醇甙 ,得率分别为2.18 %和1.07 %。High _ speed countercurrent chromatography (HSCCC) was applied to the separation and purification of resveratrol and piceid in Polygonum cuspidatum Sieb.et Zucc.. Pretreatment of samples was studied. The solvent system used for HSCCC was selected. The resveratrol and piceid yield are 2.18% and 1.07%, respectively. The purified resveratrol and piceid were identified and analyzed by TLC, HPLC, 1HNMR and MS.

关 键 词：白藜芦醇 白藜芦醇甙 色谱 虎杖 分离提纯 分析 

分 类 号：R284.2[医药卫生—中药学]