血浆中洛伐他汀含量测定的色谱-质谱法  

Determination of Lovastatin in Human Plasma by GC-MS

作　　者：郑文晖[1] 蔡葵花[1] 吴耀良[2] 

机构地区：[1]中山医科大学分子医学研究中心,广东广州510089 [2]中山医科大学附属第一医院检验部,广东广州510089

出　　处：《分析测试学报》2000年第4期69-70,共2页Journal of Instrumental Analysis

摘　　要：用色谱 -质谱法测定血浆中洛伐他汀 ,采用辛伐他汀作内标 ,以乙酸乙酯为萃取液 ,经真空抽干 ,定容进样。洛伐他汀质量浓度在0.36～48mg/L之间线性关系较好。该法灵敏度高 ,前处理简单快速 ,检出限达0.1mg/L ,相对标准偏差小于8.9 % ,回收率大于92 %。A method for the determination of lovastatin in human plasma was established by GC-MS with ion monitoring mode. Simvastatin was used as an internal standard. Sample was extracted by ethyl ace_tate, and then the extractive was used to detect after vacuum drying and fixed volume. The standard curve is linear in the range from 0.36 to 48 mg/L for lovastatin with correlation coefficient of 0.995. The method is sensitive with detection limit of 0.1 mg/L. The relative standard deviation(RSD) for intra _ day detection was found to be less than 5.0%, while RSD for inter _ day detection to be less than 8.9%. The recoveries are more than 92%.

关 键 词：洛伐他汀 色谱-质谱法 血浆 降血脂药 测定 

分 类 号：R972.6[医药卫生—药品] Q592.1[医药卫生—药学]