嗜盐嗜碱菌株20-13的生物学特性及系统发育学分析  

Biological characterization and phylogenetic analysis of the halophilic and alkaliphilic strain 20-13

作　　者：王爽[1] 王玉峰[1] 

机构地区：[1]黑龙江省农业科学院土壤肥料与环境资源研究所 黑龙江省土壤环境与植物营养重点实验室,哈尔滨150086

出　　处：《东北农业大学学报》2013年第2期42-46,共5页Journal of Northeast Agricultural University

基　　金：国家自然科学基金(41201257);黑龙江省青年基金(QC2012C062);哈尔滨市科技创新人才研究专项资金(RC2012QN002165);公益性行业(农业)科研转项(201003014-4);国家科技支撑计划子课题(2012BAD15B05)

摘　　要：对大庆盐碱土中分离到的菌株20—13进行表型特征、生理生化特性和系统发育学研究表明，菌株20-13为革兰氏阴性、能运动的杆菌，末端形成膨大的芽孢，嗜碱且中度嗜盐，能在NaCl浓度为1％～25％（W/V）的改良S-G培养基中生长，最佳生长发生在3％NaCl（W/V）；生长的温度范围20—50℃，最适为30～32℃；pH范围8．0～11．0，最适pH10．0。16SrRNA基因序列的系统进化分析表明，该菌株与厚壁菌门、碱杆菌属Alkalibacillus haloalkaliphilus的成员亲缘关系最近，相似性达99．4％，菌株20-13与Alkalibacillus haloalkaliphilus的表型特征与生理生化特性基本一致。综合分析，确定该菌株与碱杆菌属Alkalibacillushaloalkaliphilus为同一种，且与模式菌株不同的菌株。A moderately halophilic and alkaliphilic bactedal strain 20-13 isolated by endchment culture from a soil sample collected from a soda saline-alkaline soil in Daqing, China. We describe the taxonomic status of the strain 20-13 by phenotypic, physiological, biochemical and phylogenetic analysis. Morphological analysis of the cells of strain 20-13 revealed that they were Gram-negative rods and motility assays confirmed that these cells were motile. Spores are round-terminal. Strain 20-13 was able to grow at 1%-25% （WE） NaCI, with optimum growth at 3% （WN） NaCI. The temperature and pH ranges for growth 20-50 ℃ and 8.0-11.0, respectively. Optimum growth occurred at 30-32 ℃ and pH 10.0 in a modified S-G agar medium containing 10% （WN） NaCI. Phylogenetic analysis of 16S rRNA gene sequence showed that strain 20-13 was shown to belong to the genus Alkalibacillus within the phylum Firmicutes and had a close phylogenetic relationship with the specie Plkalibacillus haloalkaliphilus （99.4% sequence similarity）. In addition, strain 20-13 was consistent with specie Alkalibacillus haloalkaliphilus basically the same on phenotypic, physiological and biochemical characteristics. In summary, we suggest that strain 20-13 as different strain with the type strain of knows species AlkalibaciUus haloalkaliphilus within the genus Halornonas was proposed.

关 键 词：嗜盐菌 嗜碱菌 Alkalibacillus haloalkaliphilus 16S RRNA基因序列 

分 类 号：Q935[生物学—微生物学]