PCR-DGGE法解析牛粪厌氧发酵稳定期微生物多样性  

Analysis of microbial diversity during the stationary phase in cattle manure anaerobic fermentation with PCR-DGGE

作　　者：夏吉庆[1] 马添翼[1] 郑国香[1] 王忠江[1] 康德福[1] 王丽丽[1] 

机构地区：[1]东北农业大学工程学院,哈尔滨150030

出　　处：《东北农业大学学报》2013年第2期146-150,共5页Journal of Northeast Agricultural University

基　　金：黑龙江省自然科学基金(ZD200817);东北农业大学人才基金(2009RC49)

摘　　要：为了研究牛粪两相厌氧溢流发酵系统装置稳定产气阶段的生物菌群的生长状态及特性,使用了PCR-DGGE技术对牛粪厌氧发酵中的微生物稳定期细菌种群结构进行了研究。在发酵稳定期不同时间进行了取样,试验结果表明厌氧发酵系统细菌种群丰富,优势种群明显,群落结构较稳定。菌群具有交替生长特性,有利于系统的持续稳定产气。Polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） analysis was used to study the dynamic succession of bacterial communities of anaerobic fermentation of dairy cattle manure during the microbial stationary phase. Composts were sampled during this period. Then the results showed that the population of bacterium was abundant, the superiority of that was evident, and the increment of gas production was steady. Bacterial community was with alternating growth characteristics, that is contribute to the gas production sustained and stable.

关 键 词：PCR—DGGE 厌氧发酵 微生物 多样性 

分 类 号：S216.4[农业科学—农业机械化工程]