平板接种法监测牙科综合治疗台水路菌落总数的可行性研究  

Feasibility of plate-streaking method in monitoring total bacteria counts in dental unit waterlines

作　　者：赵红[1] 苏静[1] 刘晓勇[1] 贾淑玲[1] 

机构地区：[1]首都医科大学附属北京口腔医院,北京100050

出　　处：《中国感染控制杂志》2013年第1期16-18,23,共4页Chinese Journal of Infection Control

摘　　要：目的研究平板接种法监测牙科综合治疗台水路(DUWLs)菌落总数的可行性。方法采集某院DU-WLs系统50份水样标本,分别采用平板接种法和国家生活饮用水标准检验方法中规定的倾注平板法进行培养,比较两种方法的检测结果。结果倾注培养法和平板接种法检测DUWLs中菌落计数,差异无统计学意义(T=315.50,P>0.05);检测菌落数的合格率分别为58.00%和52.00%,差异亦无统计学意义(χ2=0.57,P>0.05)。结论平板接种法与国标倾注平板法检测DUWLs系统中的菌落总数无差异。平板接种法操作简单,易于细菌的初步鉴定,日常水质监测中可替代国标倾注平板法。Objective To study the feasibility of plate-streaking method in monitoring total bacteria counts in dental unit waterlines （DUWLs）. Methods 50 water samples from DUWLs were taken and cultured by plate streaking method and poured-plate method, colony counts of two cultured methods was compared. Results The difference in colony counts of DUWLs between poure＆plate method and plate-streaking method was not significant （T= 315.50,P〉0. 05） ; the qualified rate of water was 58. 00% and 52. 00% respectively, there was no statistical difference in the qualified rate of water detected between two methods X^22 = 0. 57,P〉0. 05）. Conclusion The total colony counts detected by plate-streaking method and poured-plate method is not different, plate-streaking method is easy to be performed, it can instead poured-plate method to monitor the colony counts in DUWLs.

关 键 词：平板接种法 倾注平板法 牙科综合治疗台水路 菌落数 医院感染 水质监测 

分 类 号：R181.32[医药卫生—流行病学]