菜用大豆胚尖转化再生体系优化研究  

Optimization for Regeneration System of Embryonic Tips of Vegetable Soybean

作　　者：胡润芳[1] 张玉梅[1] 张广庆[1] 林国强[1] 

机构地区：[1]福建省农业科学院作物研究所,福建福州350013

出　　处：《福建农业学报》2013年第1期51-54,共4页Fujian Journal of Agricultural Sciences

基　　金：农业部公益性行业(农业)科研专项(200903002);福建省科技计划重大专项(2012NZ002);福建省科技计划项目(2011R1028-3);福建省财政专项--福建省农业科学院科技创新团队建设项目(CXTD2011-14)

摘　　要：以福建省菜用大豆新品种闽豆5号的胚尖为材料,研究次氯酸钠消毒方法及不同浓度潮霉素B培养基对不定芽的诱导和生根的影响。结果表明:以10%的次氯酸钠消毒10min为宜;芽伸长阶段潮霉素B筛选压力为7mg.L-1;生根培养基不添加6-BA效果更佳,即1/2 MS盐+1/2B5有机+3%蔗糖+0.56%琼脂+1.0mg.L-1 IBA,pH 5.8。Embryonic tips from Mindou No. 5 were used in testing the effect of NaCIO on sterilization and homomycin B on screening stress for inducing of adventitious buds and root growing. The result showed that the optimal effect on sterilization was 10% NaC10 for 10 min the screening stress of hygromycin B was 7 mg L-1 at elongation stage of buds from embryonic tips. Comparing culture mediums, the rooting culture medium presented better in root growing without 6-BA, the medium consisting of 1/2 MS sah＋l/2 Bs organic matter ＋3%0 sucrose＋ 0.56% agar＋l. 0 mg. L-1 IBA, pH 5.8.

关 键 词：菜用大豆 胚尖 再生 潮霉素B 

分 类 号：S565.1[农业科学—作物学]