丙型肝炎病毒感染外周血单个核细胞对丙型肝炎的影响  

Effect of Infection of Peripheral Blood Mononuclear Cells by Hepatitis C Virus on Hepatitis C

作　　者：高勇[1] 贺永文[1] 曾令兰[1] 刘薇[1] 易建华[1] 

机构地区：[1]同济医科大学附属协和医院传染病学教研室,武汉430022

出　　处：《同济医科大学学报》2000年第5期450-452,共3页Acta Universitatis Medicinae Tongji

基　　金：国家教委启动基金!资助项目 (教留司研 1993- 36 0号 )

摘　　要：为了研究丙型肝炎病毒 (HCV)感染外周血单个核细胞 (PBMC)对丙型肝炎的影响 ,运用非同位素原位杂交法 (NISH)和链酶亲和素 -生物素 (SABC)法分别检测 2 0例慢性丙型肝炎患者 PBMC中的 HCV- RNA及其 T淋巴细胞亚群 ,同时检测这些患者的肝功能指标。结果显示 8例 (4 0 .0 % ) HCV患者的 PBMC中的 HCV- RNA呈阳性结果。HCV- RNA呈散在颗粒状或弥漫分布 ,主要位于胞浆中。 HCV- RNA阳性组和阴性组的 CD+4 T细胞比例低于正常对照组 ,CD+8T细胞比例高于正常对照组 ,CD+4 / CD+8比值下降 ;而 HCV- RNA阳性组的 CD+4 T细胞比例则又低于阴性组 ,CD+8T细胞比例高于阴性组 ,CD+4 / CD+8比值出现倒置 (P<0 .0 1)。两组的肝功能检查结果之间无显著差异 (P>0 .0 5 )。提示 HCV感染 PBMC后引起 T淋巴细胞亚群发生变化 ,使 CD+4 和 CD+8T细胞功能下降或不能很好地协调作用 ,从而成为 HCV持续感染的重要原因之一。 HCV感染To investigate the effects of infection of peripheral blood mononuclear cells (PBMCs) by hepatitis C virus (HCV) on hepatitis C, HCV RNA and T lymphocyte subsets in PBMCs were detected by non isotopic in situ hybridization (NISH) and strepatadivin biotin enzyme complex assay (SABC) respectively. Liver function was measured simultaneously. Eight (40.0 %) out of 20 patients with HCV were HCV RNA positive in PBMCs. HCV RNA signals were granule or appeared diffusely within cytoplasm. The proportion of CD + 4 T cells was lower in the patients with HCV than in the control group, the proportion of CD + 8 T cells was higher, and the ratio of CD + 4/CD + 8 were decreased significantly ( P <0.01). Moreover, the proportion of CD + 4 T cells was lower in HCV RNA positive group than in the HC RNA negative group, the proportion of CD + 8 T cells was higher, and the ratio of CD + 4/CD + 8 was reversed ( P <0.01). It was suggested that HCV infection of PBMCs could result in the change in T lymphocyte subsets, which might decrease the function of CD + 4 and CD + 8 cells or disable to incoordination well. The infection of PBMCs by HCV had a little effect on inflammation of liver.

关 键 词：丙型肝炎病毒感染 肝功能 外周血单个核细胞 

分 类 号：R512.6[医药卫生—内科学] R392.12[医药卫生—临床医学]