W/O型微乳液中超氧化物阴离子的产生──微环境中SOD酶活性测定方法研究  

Generation of superoxide anion in reverse microemulsion ─methodical study on measurement of SOD activity

作　　者：翟利民[1] 李干佐[1] 隋华[2] 袁勤生[3] 

机构地区：[1]山东大学胶体与界面化学教育部重点实验室,山东济南250100 [2]山东大学环境工程系,山东济南250100 [3]华东理工大学生物工程系,上海200237

出　　处：《分子科学学报》2000年第2期65-69,共5页Journal of Molecular Science

基　　金：国家自然科学基金!29903006

摘　　要：分别在水溶液中和 Ｗ ／Ｏ型 ＣＴＡＢ／正戊醇／异辛烷 ／水微乳液体系中研究了测定ＳＯＤ酶活性的邻苯三酚法，发现在反向微乳液中，“小水池”的低介电常数和ｐＨ抑制了底物超氧化物阴离子Ｏ２－·的产生．只有提高外加缓冲液的ｐＨ或“小水池”的水化度，才能得到所需浓度的Ｏ２－·，从而初步解决了在微乳液中ＳＯＤ作用废物的产生问题，为在微乳液中准确测定ＳＯＤ酶活性创造了条件．The pyrogallic method for determining the activity of SOD is studied in the aqueous solution and the CTAB/n -pentanol/n -octane/water microemulsion, respectively. It is found that the low pH and the dielectric constant inhibit the generation of the substrate O2-· in the reverse microemulsion which is later improved by increasing the above two factors. It solves the problem of the generation of O2- which create a new way for the determination of the activity of SOD in the microenvironment.

关 键 词：邻苯三酚 底物O2^- O\W型微乳液 SOD酶 活性测定 

分 类 号：Q554.9[生物学—生物化学]