乙酰丁香酮及共培养基pH对棉花遗传转化的影响  

Effect of Acetosyringone and Media pH on Transformation of Cotton

作　　者：陈翠娜[1] 冯德玉[1] 张新[1] 张思维[1] 代其林[1] 王劲[2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000 [2]中国农业科学院生物技术研究所,北京100084

出　　处：《上海交通大学学报（农业科学版）》2013年第1期18-22,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：转基因生物新品种培育重大专项(2009ZX08009-091B);国家自然科学基金(30871555);教育部新世纪优秀人才支持计划(NCET-08-0940);四川省教育厅(09ZA034);西南科技大学博士研究基金(11zx7104)

摘　　要：以陆地棉种质系珂字棉312为材料,探讨了在共培养基添加乙酰丁香酮(AS)同时调节pH值对抗性愈伤组织诱导率、正常体细胞胚数以及Kan抗性苗获得率的影响。结果表明,在共培养阶段的乙酰丁香酮浓度与pH均对棉花遗传转化有明显的影响。当乙酰丁香酮的浓度为100μmol/L、pH值为5.6时,棉花的遗传转化率最高。其抗性愈伤组织的诱导率达到72.96%,Kan抗性苗获得率可达到8.96%;对转化再生植株进行PCR检测,约52.07%的Kan抗性苗呈阳性。Using hypocotyl of Coker 312 （Gossypium hirsuturnL. ） as the experimental material,this study investigated the effect of acetosyringone （AS） concentration and pH in the co-culture medium on the resistant calli-inducing rate,number of somatic embryos and transformation efficiency. The results showed that the transformation efficiency was significantly affected by acetosyringone （AS） concentration and pH in the co-culture. The optimized acetosyringone concentration was 100μmol/L and the appropriate pH was 5.6, which can significantly improve the efficiency of genetic transformation in cotton. The rate of resistant calli was 72.96% and the harvesting proportion of Kan＋ regenerated plants reached 8. 960/oo. Regenerated plants were confirmed by PCR and about 52.07% regenerated plants were proven to be resistant.

关 键 词：乙酰丁香酮 PH 根癌农杆菌 棉花 

分 类 号：Q943.2[生物学—植物学]