转燕麦噬酸菌蛋白激发子基因flagellin的链霉工程菌株构建及鉴定  

Construction and Identification of Streptomyces lydicus A01 Transformed with Elicitor Gene Flagellin from Acidovorax avenae Strain N1141

作　　者：吴琼[1] 林振亚[1] 李莹莹[1] 李雅乾[1] 高士刚[1] 陈捷[1] 

机构地区：[1]上海交通大学农业与生物学院,农业部都市农业(南方)重点开放实验室,上海200240

出　　处：《上海交通大学学报（农业科学版）》2013年第1期56-60,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科委重点项目(09391910900);国家863项目(2011AA10A205);948项目(2011-G4)

摘　　要：利迪链霉菌A01可以产生大量的抗生素——纳他霉素,其通过结合病原真菌细胞膜上的甾醇分子而起到抑制病原菌生长的作用。燕麦噬酸菌的鞭毛蛋白组分FLAGELLIN可以诱导植物抗性,激发活性氧及水杨酸防御反应途径。本研究将燕麦噬酸菌蛋白激发子基因flagellin克隆,接合转移入利迪链霉菌A01中,使工程菌增加诱导植物抗性的功能。PCR验证表明:成功获得了含flagellin基因的阳性工程菌株,证明通过链霉工程菌构建可实现抗生与诱抗的协同作用,预计会提高防治植物病害的效果。Streptomyces lydicus A01 produces natamycin, which inhibit the growth of fungi through specifically binding to ergosterol of fungal cell membrane. Flagellin, expressed by Acidovorax avenae strain Nl141, possesses the abilities of inducing reactive oxygen species（ROS）and activating salicylic acid（SA）signal pathway in plants. In this study, we cloned flagellin gene from Acidovorax avenae strain Nl141, transformed it into S. lydicus A01 using conjugation methods, and detected it by PCR analysis. The results showed that positive engineered strain containing flagellin gene was successfully established and the construction of Streptomyces lydicus with flagellin gene could achieve the synergic effects of anti-fungus and induced resisance. This technology showed potential advantage of promoting anti-fungus function in plants.

关 键 词：利迪链霉菌A01 燕麦噬酸菌flagellin基因 工程菌株 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]