生物技术培养银杏发根中的黄酮分析研究  

The Study and Analysis of Flavonoids in the Cultured Ginkgo Root Hairs by Biological Technique

作　　者：颜金莲[1] 罗保生[1] 纪竹生[1] 孙天恩[1] 

机构地区：[1]武汉大学分析测试科学系,武汉430072

出　　处：《分析科学学报》2000年第4期320-325,共6页Journal of Analytical Science

摘　　要：本文研究了银杏发根中黄酮含量的高效液相色谱 ( HPL C)分析方法 ,改进了样品预处理过程 ,实行提取、水解同步进行 ,并与天然银杏树叶中的黄酮进行了对照 ,分析结果表明 ,采用生物技术培养的银杏发根中确实含有银杏黄酮。A high performance liquid chromatographic method has been developed to determine the flavonoids content in cultured Ginkgo root hairs. The pretreatment procedure of samples was improved, in which the extraction and the hydrolysis were carried out simultaneously. The results were compared to those in nature Ginkgo biloba leaves, which indicated that there were flavonoids in cultured Ginkgo root hairs by biological technique.

关 键 词：黄酮 银杏发根 生物技术培养 分析 HPLC 

分 类 号：R284.1[医药卫生—中药学]