大黄酸对高脂饮食诱导的大鼠非酒精性脂肪性肝病的防治作用  

Protective Effects of Rhein on Nonalcoholic Fatty Liver Disease in Rats Fed with High Fat Diet

作　　者：岑柏春 张谈 袁建芬 陆新烈 徐建立 施军平[2] 荀运浩[2] 

机构地区：[1]慈溪市中医医院,浙江慈溪315300 [2]浙江中医药大学附属杭州第六医院

出　　处：《中华中医药学刊》2013年第3期545-547,I0005,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划项目(2010ZA112);浙江省自然科学基金资助项目(Y2091200;Y2091159)

摘　　要：目的:研究大黄酸对大鼠非酒精性脂肪肝(NAFLD)的防治作用及其部分机制。方法:采用高脂饲料12周喂养建立大鼠NAFLD模型;50只SD大鼠随机分为5组:正常对照组、模型组、大黄酸低、中、高剂量[100,200,400 mg/(kg.d)]组(每组10只)。给药4周后处死大鼠,计算肝脏指数,观察肝组织病理形态学变化,全自动生化仪检测血清ALT、AST、FFA、LDL-C、HDL-C、TG和TC含量;比色法测定肝组织MDA、GSH-PX水平;Real-time PCR检测肝脏组织PPAR-γmRNA表达。结果:与模型组比较,大黄酸给药可使大鼠肝组织脂肪变性明显减轻,肝脏指数明显降低;各给药组血清ALT、AST、FFA、TG、TC和肝脏MDA水平降低,肝脏GSH-PX活力升高(P<0.05或0.01);模型组肝脏PPAR-γmRNA表达明显升高(P<0.01),大黄酸干预组则明显降低其表达(P<0.01)。结论:大黄酸对大鼠NAFLD具有较好的防治作用,其机制可能与清除氧自由基、减少脂质过氧化产物和改善脂质代谢紊乱有关。Objective：To investigate the beneficial effects of Rhein on nonalcoholic fatty liver disease（NAFLD）in rats fed with high fat diet and to explore the possible mechanisms.Methods：50 SD rats were divided into normal group,control group,and low,medium,high dose of Rhein [100,200,400 mg/（kg·d）,respectively]group randomly（10 rats in each group）.NAFLD was induced by a formula of high fat（HF）diet.At the treatment time of 4 weeks later,rats were sacrificed,and hepatic index was calculated.Histologic changes were observed and compared between these groups.Serum levels of ALT,AST,free fatty acids（FFA）,LDL-C,HDL-C,TG and total cholesterol（TC）were detected by automatic biochemical analyzer,hepatic contents of malondialdehyde（MDA）and glutathione peroxidase（GSH-PX）were evaluated by colorimetric technique,hepatic expression of peroxisome proliferator-activated receptor（PPAR）-γ mRNA was detected by real-time PCR.Results：Comparing with that of control group,liver steatosis in those Rhein groups were alleviated obviously,and hepatic indexes were decreased also.A consistent decline of serum levels of ALT,AST,FFA,LDL-C,TG,TC and hepatic MDA,and increase of hepatic GSH-PX were obtained in all of the three Rhein groups（P〈0.05 or P〈0.01）.As for the hepatic expression of PPAR-γ mRNA,HF diet resulted in a marked increase but improved effectively by Rhein（P〈0.01 for all）.Conclusions：Rhein improves NAFLD in a rat model of HF diet,the effect is contributed partly to the mitigated oxidative stress and improvement of lipid metabolism.

关 键 词：大黄酸 非酒精性脂肪肝 大鼠 自由基 过氧化物酶体增生物激活受体 

分 类 号：R285.5[医药卫生—中药学]