腺苷A_(2A)受体在CIA小鼠电针抗炎效应中的作用机制研究  

Mechanism Research of Adenosine A2A Receptor on Anti-inflammatory Effects of Electric Acupuncture in CIA Mice

作　　者：李晓佩[1] 李方[2] 李芝慧[1] 李营营[1] 李奇辉[1] 谢文霞[2] 叶天申[2] 

机构地区：[1]温州医学院,浙江温州325000 [2]温州医学院附属第一医院,浙江温州325000

出　　处：《中华中医药学刊》2013年第3期591-594,I0009,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医风湿病重点专科项目(200719956)

摘　　要：目的:建立稳定的C57BL/6小鼠胶原性关节炎模型,初步探讨电针抗炎效应与腺苷A2A受体(A2AR)的关系。方法:用鸡Ⅱ型胶原与完全弗氏佐剂在小鼠尾根部皮内注射免疫造模。选取造模成功的40只小鼠随机分为模型组、电针组、电针加A2AR拮抗剂组和A2AR激动剂组。初次免疫后5周开始电针治疗,针刺前腹腔注射拮抗剂或激动剂,每日1次,连续干预14天。观察干预后小鼠肿瘤坏死因子α(TNF-α)、膝踝关节组织病理和X线的变化。结果:①电针组和A2AR激动剂组小鼠的TNF-α含量、病理切片评分和X线评分明显低于模型组,差异有统计学意义(P<0.01),电针组和A2AR激动剂组比较差异无统计学意义(P>0.05);②电针加A2AR拮抗剂组小鼠的的以上指标与模型组比较,差异无统计学意义(P>0.05)。结论:根据本研究结果推测:电针可能增加内源性腺苷的含量,通过激动A2AR而发挥抗炎效应,这可能是其治疗类风湿关节炎的作用机制之一。Objective：To observe the therapeutic effect of acupuncture for collage-induced arthritis（CIA） in C57BL/6 mice and discuss its underlying mechanism with adenosine A2A receptor（A2AR）.Methods：C57BL/6 mice were immunized by intradermal injection at the base of the tail with chick collagenⅡand complete Freund adjuvant emulsion.A total of 40 mice were randomized into model,EA,EA＋SCH58261 and CGS21680 groups,with 10 cases in each group.Acupuncture was carried out from the 5th week on after the initial immunization,A2AR antagonist or agonist was transperitoneally administered before acupuncture,once a day for 2 weeks.After treatment with acupuncture and drug,serum TNF-α level,joint pathology and X-ray were examined.Results：Compared with the model group,significant diminutions were found between the EA and CGS21680 groups in TNF-α level,pathological score and radiographic score（P〈0.01）,while no significant differences were found between the two groups（P〈0.05）.In comparison with the model group,the above indices of the EA＋SCH58261 group were not changed significantly（P〈0.05）.Conclusion：According to the results of this study：acupuncture may increase endogenous adenosine content,exerting anti-inflammatory effects by exciting A2AR,which may be one of the main mechanism of acupuncture to treat RA.

关 键 词：C57BL 6小鼠 胶原性关节炎 抗炎效应 电针 腺苷A2A受体 

分 类 号：R245.97[医药卫生—针灸推拿学]