反相高效液相色谱法测定祛瘀清胰颗粒中牛磺熊去氧胆酸的含量  

Determination of Tauroursodeoxycholic Acid in Quyuqingyi Granule by Rp-HPLC

作　　者：于艳[1] 于世杰[1] 修丽丽[1] 孙科峰[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2013年第3期621-623,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：沈阳市科技局资助项目(F10-149-9-12)

摘　　要：目的:建立反相高效液相色谱法测定祛瘀清胰颗粒中牛磺熊去氧胆酸含量的方法。方法:采用高效液相色谱法。色谱柱:Agilent TC-C18(4.6 mm×250 mm,5μm);流动相乙腈-0.5%磷酸水溶液,梯度洗脱;检测波长203nm;流速1.0 mL.min-1;柱温30℃。结果:牛磺熊去氧胆酸在0.259～5.180μg范围内线性关系良好,r=0.9999,平均回收率99.2%,RSD为2.3%。结论:本方法简便、准确、灵敏度高,重复性好,可有效控制祛瘀清胰颗粒的质量。Objective：To establish a method for determining the content of TUDCA in Quyuqingyi granule by Rp - HPLC. Methods ：The HPLC method was used. The analytical column was Agilent TC - Cls （4.6 mm ×250 mm,5μm）.The mobile phase was by gradient elution with mixture liquid of acetonitrile and 0.5% （v/v） phosphoric acid in water. The UV detector wavelength was set at 203nm, the flow rate was 1.0 mL ·min^-1, and the column temperature was 30 ℃. Results ： The calibration curve was linear（ r = 0.9999） in the range of 0. 259 - 5.180 μg for TUDCA. The average recovery of the method was 99.2% with RSD 2.3%. Conclusion ：The method is simple, accurale, sensitive and stable,and can be used for the quality control of Quyuqingyi granule.

关 键 词：高效液相色谱 祛瘀清胰颗粒 牛磺熊去氧胆酸 

分 类 号：R284.1[医药卫生—中药学]