苍术苷的致突变AMES试验研究  

Ames Test of Atractyloside

作　　者：杨桂蓉[1] 喻凯[1] 陆彩鹏[1] 周蓉[1] 邓喜[1] 王明奎[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《中华中医药学刊》2013年第3期637-638,共2页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:应用Ames试验检测系统检测苍术苷的致突变活性。方法:采用组氨酸营养缺陷型鼠伤寒沙门杆菌TA97,TA98,Tal00,Tal02标准测试菌株,在有和无代谢活化剂下对受试药品0.8～250μg·皿-1共5个浓度组进行测试。结果:无S9活化系统条件下,各菌株回复突变菌落数未见明显增多。有S9活化系统条件下,4～250μg·.皿-1测试浓度范围内,苍术苷对TA98测试菌株诱发产生的回变菌落数与空白对照组相比均达到两倍以上。结论:苍术苷4～250μg·皿-1测试浓度范围内,对TA98有间接致突变活性。Objective：To study the mutagenic effects of atractyloside by Ames assay in vitro.Methods：In the presence and absence of S9 activator,the mutagenic activities of atractyloside at the concentrations within 0.8-250 μg/mL were observed in the TA series of typhoid salmonella bacillus strains TA97,TA98,Tal00 and Tal02.Results：All kinds of series colnies were not increased in absence of S9 activator.The mutated clump count of atractyloside at the concentrations within 4~250 μg/mL about TA98 were above double compared with that in negative control.Conclusion：The mutagenic activities of atractyloside at the concentrations within 4~250 μg/mL about TA98 were observed indirectly.

关 键 词：苍术苷 AMES试验 致突变活性 

分 类 号：R285.5[医药卫生—中药学]