不同盐浓度影响中药外洗方中小檗碱透皮吸收的体外实验研究  

Different salinity influenced the percutaneous absorption of Berberine in washout application of traditional Chinese medicine in vitro

作　　者：黄开远[1] 温泉[1] 黎晖[1] 杨开磊 杨伟华 秦红芳[1] 陈静[1] 曾玲[1] 

机构地区：[1]广州中医药大学,广东广州510006 [2]广东省多品种盐公司,广东广州510360

出　　处：《时珍国医国药》2013年第2期273-275,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81072908);广东省科技计划项目(No.2009B030801283)

摘　　要：目的不同盐浓度影响中药外洗方中小檗碱透皮吸收的体外实验研究。方法建立小檗碱皮肤渗透液的高效液相色谱检测法,利用昆明小鼠腹部皮肤,以Franz扩散池研究盐对小檗碱的透皮能力,研究含0%、0.1%、0.9%、2.7%、3.6%不同盐浓度外洗方中小檗碱的透皮吸收效果。结果小檗碱在体外可以通过透皮吸收,含0.9%和2.7%盐在3～8h的促渗作用明显优于含0%盐组。通过单位时间透皮量比较,2～3 h可能是药物的最佳透皮吸收时间。3.6%盐组在1～4 h有促渗作用,但是4～8 h则抑制小檗碱的透皮吸收。结论 0.1%、0.9%、2.7%盐均能够增加小檗碱的透皮吸收,其中0.9%盐能显著增加小檗碱的透皮量和透皮速率,2～3 h可能是药物的最佳吸收时间。ObjectiveTo study the effect of different salinity on percutaneous absorption of Berberine in washout application of traditional Chinese medicine in vitro. MethodsThe percutaneous absorption of Berberine was performed through excised Kunming mouse skin of abdomen in the Franz diffusion pond.Different salinity（groups of 0%,0.1%,0.9%,2.7%,3.6% salt respectively） of physic liquor up the Franz diffusion pond permeated the skin into the reception liquor.Berberine in the reception liquors was determined by HPLC in 1,2,3,4,6 h,and 8 h. ResultsBerberine in the reception liquors of 0.9% and 2.7% salt groups were significantly higher than that of the 0% salt group in 3~8 h.The group of 3.6% salt enhanced the percutaneous absorption of the berberine in 1~4 hour,and then inhibited that in 4~8 h. ConclusionThe group of 0.9%,0.1%,and 2.7% salt improved the percutaneous absorption of the Berberine in 1~8h,and the effect of 0.9% salt on the percutaneous absorption of the Berberine might be the most significance,The time of percutaneous absorption reached a plateau at 2 to 3 hours.

关 键 词：盐 小檗碱 透皮吸收 外洗液 

分 类 号：R284.2[医药卫生—中药学]