尼古丁对小鼠镇痛作用的研究  

Study on Antalgic Effect of Nicotine in Mice

作　　者：刘真[1] 马晓丽[2] 夏甜甜[1] 孙海基[1] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]山东大学附属济南市中心医院,山东济南250014

出　　处：《时珍国医国药》2013年第2期279-280,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(No.81272588);山东省自然科学基金面上项目(No.2012ZRA01007);山东省高等学校科技计划(No.J11LC14)

摘　　要：目的探讨尼古丁的镇痛作用。方法采用小鼠热板法、小鼠醋酸扭体法和小鼠热水甩尾法,观察腹腔注射0.25,0.5,1和2.5 mg/kg四种剂量的尼古丁对疼痛的影响。结果 2.5 mg/kg剂量的尼古丁可使热板所致小鼠舔足反应潜伏期延长,与给药前相比,潜伏期提高率为99.5%;1 mg/kg剂量的尼古丁可使醋酸所致小鼠扭体潜伏期延长,扭体次数减少,扭体次数的抑制率为53.0%;1 mg/kg剂量的尼古丁可使热水所致小鼠甩尾潜伏期延长,与给药前相比,潜伏期提高率为44.73%。结论尼古丁具有镇痛作用,且其镇痛作用与剂量相关。ObjectiveTo explore the antalgic effect of nicotine. MethodsThe antalgic effect was observed by mice writhing test,mice Tail-immersion test and hot-plate test,being given four concentrations（0.25,0.5,1 and 2.5 mg/kg）of nicotine via abdominal injection. ResultsThe results indicated that ①In hot-plate test,compared with the control group,the 2.5 mg/kg dose of nicotine resulted in a significant increase in the mice＇s pain threshold,and the maximum of analgesic ratio was 99.5% ②Compared with the control group,the 2.5 mg/kg dose of nicotine significantly prolonged mice＇s writhing latency and remarkedly decreased writhing responses,the maximum of inhibitory ratio was 53%;③ In mice tail-immersion test,Tail-curl latency in the 1mg/kg nicotine injected group was observably prolonged and the maximum of analgesic ratio was 44.7%. ConclusionThese results showed that nicotine has dose-related antalgic effects.

关 键 词：尼古丁 镇痛 热板法 醋酸扭体法 热水甩尾法 

分 类 号：R285.5[医药卫生—中药学]