脾阳虚大鼠下丘脑星形胶质细胞GFAP和小胶质细胞OX42的表达及大建中汤的干预作用  

Effect of Dajianzhong decoction on the expression changes of GFAP and OX42 in hypothalamus in Spleen Yang Deficiency rats

作　　者：王慧[1] 武静[1] 陈继婷[1] 

机构地区：[1]贵阳中医学院基础医学院,贵州贵阳550002

出　　处：《时珍国医国药》2013年第2期304-305,共2页Lishizhen Medicine and Materia Medica Research

基　　金：贵州省自然科学基金资助(黔科合J字[2010]2192号)

摘　　要：目的观察脾阳虚症大鼠下丘脑星形胶质细胞和小胶质细胞表达的改变,以及大建中汤对脾阳虚大鼠下丘脑星形胶质细胞和小胶质细胞表达的影响,探讨下丘脑神经胶质细胞在脾阳虚症发病机制中的作用和大建中汤可能的作用环节。方法采用饮食失节伤脾气,劳倦过度伤脾气及苦寒泻下伤脾阳的方法,建立脾阳虚模型。实验分为正常对照组,模型组,大建中汤治疗组。采用免疫组织化学染色法对下丘脑胶质原纤维酸性蛋白(GFAP)和特异性标记小胶质细胞的OX42抗体进行染色,观察与内脏活动密切相关的下丘脑组织中星形胶质细胞GFAP和小胶质细胞OX42表达的变化。结果与正常对照组相比,脾阳虚模型组大鼠下丘脑GFAP与OX42表达增强,阳性细胞数增多,差异具有统计学意义(P<0.01);大建中汤高剂量组GFAP与OX42表达与模型组相比明显下降,具有显著性差异(P<0.01)。结论脾阳虚能激活下丘脑星形胶质细胞和小胶质细胞,胶质细胞的激活可能是脾阳虚症大鼠模型形成的神经机制之一,大建中汤可能通过作用于神经胶质细胞来达到其干预脾阳虚症的作用。ObjectiveTo investigate the expression changes of GFAP and OX42 in hypothalamus in Spleen Yang Deficiency rats,and the possible regulating mechanism of hypothalamic astrocytes and microglia cells in rats with Spleen Yang Deficiency after administrated Dajianzhongtang decoction. MethodsSpleen Yang Deficiency rat model was established.Rats were divided into control group,model group,Dajianzhong decoction group.The change of the hypothalamic expression of glial fibrillary acidic protein（GFAP） and OX42 expression was detected by using immunohistochemical staining. ResultsCompare with control group,model group showed significantly higher levels（P0.01） of GFAP and OX42 positive cells.Treatment animals receiving a high dose of Dajianzhong decoction showed significantly lower levels（P0.01） of GFAP and OX42 positive cells than model group animals. ConclusionSpleen Yang Deficiency in the rat model activates hypothalamic astrocytes and microglia;a Dajianzhong decoction has a counteractive effect by reducing hypothalamic GFAP and OX42 expression.

关 键 词：大建中汤 脾阳虚 下丘脑 胶质细胞 

分 类 号：R285.5[医药卫生—中药学]